Detection of interaction between HIPK3 and SF-1 by coimmunoprecipitation. After expression of SF-1-HA and Flag-sHIPK3 (aa 159 to 1191) in H1299 cells, the HIPK3 protein complex was immunoprecipitated with anti-Flag antibody or by direct loading to the gel (input). Western blotting was then performed to detect SF-1-HA and Flag-sHIPK3.

Polyclonal Rabbit anti-Human HIPK3 / FIST Antibody (aa1127-1156, IHC, WB)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:	Human
Format:	Liquid

$401.00each

Catalog Number: LS-C99903-400

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Quantity

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Overview

Description: FIST antibody LS-C99903 is an unconjugated rabbit polyclonal antibody to human FIST (HIPK3) (aa1127-1156). Validated for IHC and WB.
Application: IHC, IHC-P, WB
Reactivity: Hu
Predicted Reactivity: Ms, Rt
Intended Use: RUO
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: ANPK | FIST | FIST3 | Homolog of protein kinase YAK1 | DYRK6 | HIPK3 | PKY | YAK1
Recommended Dilution: IHC-P: 1:50 - 1:100
Recommended Dilution: WB: 1:1000
UniProt Number: Q9H422
NCBI GenBank: NM_005734, NP_005725.3
SwissProt: Q9H422

Target

Target: Human HIPK3 / FIST
Antigen Species: Hu
Epitope: This HIPK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1127-1156 amino acids from the C-terminal region of human HIPK3.
Gene Name: HIPK3

Antibody

Host Species: Rb
Clonality: Polyclonal
Origin Species: Hu

Immunogen

Immunogen Type: SP-Lin

Format

Conjugate/Label: Unconj
Format: Liquid
Formulation: PBS, 0.09% Sodium Azide
Volume: 400 Microliter
Preservative: Yes
Purification Method: AS Ppt

Storage & Handling

Storage Conditions: Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Recommended Storage Buffer: PBS