LSBio
HDAC1 Antibody (aa450-482)

Immunohistochemistry of rabbit Anti-HDAC-1 Antibody. Tissue: human prostate cancer tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: HDAC-1 antibody at 1:500 for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: HDAC-1 is nuclear. Staining: HDAC-1 precipitated purple with blue counterstain.

HDAC1 Antibody (aa450-482)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Western Blot
Reactivity:Human
Format:Liquid
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$311.00each
Catalog Number: LS-C745294-25

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Overview

Description
HDAC1 antibody LS-C745294 is an unconjugated rabbit polyclonal antibody to human HDAC1 (aa450-482). Validated for ELISA, IF, IHC and WB.
Application
ELISA, IF, IHC, WB
Reactivity
Hu
Predicted Reactivity
Ms, NHP, Rt
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
HDAC1 | Histone deacetylase 1 | GON-10 | HD1 | RPD3 | RPD3L1
Recommended Dilution: ELISA
1:10000 - 1:50000
Recommended Dilution: IF/ICC
10 ug/ml
Recommended Dilution: IHC
1:200 - 1:1000
Recommended Dilution: WB
1:1000 - 1:5000
UniProt Number
Q13547
SwissProt
Q13547

Target

Target
Human HDAC1
Antigen Species
Hu
Epitope
A BLAST analysis was used to suggest reactivity with this protein from human, mouse, rat and chimpanzee sources based on 100% homology for the immunogen sequence. Cross reactivity may occur with HDAC-1 from bovine (82% homology) and chicken (80% homology) sources. Cross reactivity with HDAC-1 homologues from other sources has not been determined.
Gene Name
HDAC1

Antibody

Host Species
Rb
Clonality
Polyclonal
Isotype
IgG
Origin Species
Hu

Immunogen

Immunogen
Anti-HDAC-1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region near amino acids 450-482 of Human HDAC-1.
Immunogen Type
SP-Lin

Format

Conjugate/Label
Unconj
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.1% Sodium Azide
Concentration
1 mg/mL
Volume
25 Microliter
Preservative
Yes
Purification Method
AP

Storage & Handling

Storage Conditions
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.