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Antibodies > H3F3B > LS-C675332
 
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Catalog Number Size Price
LS-C675332-50 50 µl $316 
LS-C675332-100 100 µl $386 
H3F3B Antibody - Immunocytochemistry analysis diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
H3F3B Antibody - Western Blot Positive WB detected in:Hela whole cell lysate, 293 whole cell lysate, NIH/3T3 whole cell lysate All Lanes:Phospho-Histone H3 (T3) antibody at 1.41µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 16 KDa Observed band size: 16 KDa
H3F3B Antibody - Overlay histogram showing Hela cells stained (red line) at dilution of 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal Goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C.The Secondary antibody used was FITC Goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
H3F3B Antibody - Immunocytochemistry analysis diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
H3F3B Antibody - Western Blot Positive WB detected in:Hela whole cell lysate, 293 whole cell lysate, NIH/3T3 whole cell lysate All Lanes:Phospho-Histone H3 (T3) antibody at 1.41µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 16 KDa Observed band size: 16 KDa
H3F3B Antibody - Overlay histogram showing Hela cells stained (red line) at dilution of 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal Goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C.The Secondary antibody used was FITC Goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
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Monoclonal Rabbit anti‑Human H3F3B Antibody (WB) LS‑C675332

Monoclonal Rabbit anti‑Human H3F3B Antibody (WB) LS‑C675332

Submit Review LSBio Guarantee
Antibody:
H3F3B Rabbit anti-Human Monoclonal Antibody
Application:
ICC, WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$316
LS-C675332-50
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
H3F3B Rabbit anti-Human Monoclonal Antibody
Application:
ICC, WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
H3F3B antibody LS-C675332 is an unconjugated rabbit monoclonal antibody to human H3F3B. Validated for ELISA, Flow, ICC and WB.
Target
Human H3F3B
Synonyms
H3F3B | H3.3B | Histone H3.3
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Monoclonal
Conjugations
Unconjugated
Purification
Affinity chromatography
Modifications
Unmodified
Immunogen
A synthesized peptide
Applications
  • ICC
  • Western blot
  • Flow Cytometry
  • ELISA
Presentation
PBS, pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About H3F3B
Q6NXT2 NM_005324 NP_005315.1

Publications (0)

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H3F3B Antibody LS‑C675331
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, ELISA
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See all H3F3B Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 5/18/2024
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