Detection of Human and Mouse Histone H2AX by Western Blot. Samples: Nuclear extract from human 293T, human G-361, and wild-type (+/+) or H2AX knockout (-/-) mouse embryonic fibroblasts. Antibody: Affinity purified rabbit anti-H2AX antibody used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 5 seconds.
H2AFX / H2AX Antibody (C-Terminus)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Liquid |
$301.00each
Catalog Number: LS-C287020-10
Catalog Number: LS-C287020
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- Description
- H2AX antibody LS-C287020 is an unconjugated rabbit polyclonal antibody to H2AX (H2AFX) (C-Terminus) from human. It is reactive with human and mouse. Validated for IHC and WB.
- Application
- IHC, WB
- Reactivity
- Hu, Ms
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- H2AFX | H2AX | Histone H2A.x | H2A.X | H2A histone family, member X | H2AX histone
- Recommended Dilution: IHC
- 1:500 - 1:2000
- Recommended Dilution: WB
- 1:2000 - 1:10000
- UniProt Number
- P16104
- NCBI GenBank
- NM_002105, NP_002096.1
- SwissProt
- P16104
Target
- Target
- Human H2AFX / H2AX
- Antigen Species
- Hu
- Epitope
- The epitope recongized by A300-083A maps to the C-terminus of human histone H2AX using the numbering given for Swiss-Prot entry P16104 (GeneID 3014).
- Gene Name
- H2AX
Antibody
- Host Species
- Rb
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- A portion of GeneID: 3014 corresponding to amino acids near the C-Terminus of UniprotID: P16104
Format
- Conjugate/Label
- Unconj
- Format
- Liquid
- Formulation
- Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
- Preservative
- Yes
- Purification Method
- AP
Storage & Handling
- Storage Conditions
- Store at 2-8°C for up to one year.
- Recommended Storage Buffer
- Tris-citrate