LSBio
Polyclonal Goat Anti-Rat IgG Secondary Antibody (AP, IHC, WB)

Western Blot of Alkaline Phosphatase Conjugated Goat anti-Rat IgG antibody. Lane 1: Rat IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Alkaline Phosphatase rat secondary antibody at 1:1000 for 60 min at RT. Block: MB-070 for 30 min RT. Predicted/Observed size: 55 kDa, 28 kDa for Rat IgG. Other band(s): none. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Polyclonal Goat Anti-Rat IgG Secondary Antibody (AP, IHC, WB)

Secondary AntibodyLSBio Guarantee
Antibody:Goat Polyclonal
Applications:Dot Blot, Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:Rat
Format:Liquid
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$360.00each
Catalog Number: LS-C60997-1

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Overview

Description
Rat IgG antibody LS-C60997 is an AP-conjugated goat polyclonal antibody. Validated for ELISA, IHC and WB.
Application
DB, ELISA, IHC, WB
Reactivity
Rt
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Secondary Antibodies
Recommended Dilution: ELISA
1:50000 - 1:90000
Recommended Dilution: IHC
1:200 - 1:1000
Recommended Dilution: WB
1:500 - 1:2500

Target

Target
Rat IgG
Target Heavy Chain
IgG
Antigen Species
Rt
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Rat IgG and Rat Serum.

Antibody

Host Species
Gt
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Rat IgG whole molecule
Immunogen Type
Native

Format

Conjugate/Label
AP
Format
Liquid
Formulation
10 mg/mL BSA (Protease and Ig free), 0.05 M Tris-Chloride, pH 8.0, 0.001 M MgCl2, 0.0001 M ZnCl2, 0.15 M NaCl, 0.1% Sodium Azide, 50% (v/v) Glycerol
Concentration
1 mg/mL
Preservative
Yes
Purification Method
AP

Storage & Handling

Storage Conditions
Store at 4°C. Do not freeze.
Recommended Storage Buffer
0.05 M Tris-Chloride|0.15 M NaCl

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.