LSBio
Goat Anti-Rabbit IgG Secondary Antibody (HRP)

RABBIT IgG (H&L) Antibody Peroxidase Conjugated Western Blot. Western blot of Peroxidase Conjugated Goat Anti-Rabbit Antibody Lane 1: HeLa Whole Cell Lysate Lane 2: NIH/3T3 Whole Cell Lysate Load: 10 ug per lane Primary antibody: Beta Actin Antibody at 1:1000 for overnight at 4C Secondary antibody: Peroxidase Conjugated Goat Anti-Rabbit Antibody at 1:40000 for 30 min at RT Block: MB-070 for 30 min at RT Predicted/Observed size: 42 kD, 42 kD. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Goat Anti-Rabbit IgG Secondary Antibody (HRP)

Secondary AntibodyLSBio Guarantee
Antibody:Goat Polyclonal
Applications:Dot Blot, Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:Rabbit
Format:Lyophilized
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$324.00each
Catalog Number: LS-C60884-2

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Overview

Description
Rabbit IgG antibody LS-C60884 is an HRP-conjugated goat polyclonal antibody. Validated for ELISA, IHC and WB. Cited in 2 publications.
Application
DB, ELISA, IHC, WB
Reactivity
Rb
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Secondary Antibodies
Recommended Dilution: ELISA
1:150000 - 1:250000
Recommended Dilution: IHC
1:500 - 1:2500
Recommended Dilution: WB
1:10000 - 1:20000

Target

Target
Rabbit IgG
Target Heavy Chain
IgG
Antigen Species
Rb
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum.

Antibody

Host Species
Gt
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Rabbit IgG whole molecule
Immunogen Type
Native

Format

Conjugate/Label
HRP
Format
Lyophilized
Formulation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
Preservative
Yes
Purification Method
AP

Storage & Handling

Storage Conditions
Store lyophilized at 4°C. Following resuspension aliquot and store at -20°C. Avoid freeze-thaw cycles and dilute immediately before use.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl
Gingival tissue human beta-defensin levels in relation to infection and inflammation. Meltem Özdemir, Feriha Caglayan, Floris J Bikker, Pirkko Pussinen, Eija Könönen, Nermin Yamalik, Mervi Gürsoy, Dareen Fteita, Kamran Nazmi, Güliz N Güncü, Milla Pietiäinen, Mimmi Tolvanen, Ulvi Kahraman Gürsoy. Journal of clinical periodontology. 2020 March;47:309-318. PubMed: 31799742

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