LSBio
Goat Anti-Rabbit IgG Secondary Antibody (Biotin)

ELISA results of purified Goat F(ab')2 Anti-Rabbit IgG Antibody Biotin Conjugated Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins tested against purified Rabbit IgG (Purple Line). Each well was coated in duplicate with 1.0 µg of Rabbit IgG. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer

Goat Anti-Rabbit IgG Secondary Antibody (Biotin)

Secondary AntibodyLSBio Guarantee
Antibody:Goat Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunoblot, Immunohistochemistry (general), Other, Western Blot
Reactivity:Rabbit
Format:Lyophilized
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$368.00each
Catalog Number: LS-C61484-500

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Overview

Description
Rabbit IgG antibody LS-C61484 is a biotin-conjugated goat polyclonal antibody. Validated for ELISA, IE, IHC and WB.
Application
ELISA, IB, IHC, Other, WB
Reactivity
Rb
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Secondary Antibodies
Recommended Dilution: ELISA
1:20000 - 1:100000
Recommended Dilution: IHC
1:1000 - 1:5000
Recommended Dilution: WB
1:2000 - 1:10000

Target

Target
Rabbit IgG
Target Heavy Chain
IgG
Antigen Species
Rb
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.

Antibody

Host Species
Gt
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Anti-Rabbit IgG F(ab')2 fragment was produced by repeated immunization with Rabbit IgG whole molecule in goat.
Immunogen Type
Native

Format

Conjugate/Label
Biotin
Format
Lyophilized
Formulation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
AP

Storage & Handling

Storage Conditions
Store at 4°C.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.