LSBio
Polyclonal Goat Anti-Mouse IgG Secondary Antibody (Atto 647N, IF, WB)

Western Blot of Goat anti-Mouse IgG Pre-Adsorbed ATTO 647N Conjugated Secondary Antibody. Lane 1: Mouse IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: ATTO 647N goat secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Mouse IgG. Other band(s): None.

Polyclonal Goat Anti-Mouse IgG Secondary Antibody (Atto 647N, IF, WB)

Secondary AntibodyLSBio Guarantee
Antibody:Goat Polyclonal
Applications:Immunofluorescence, Other, Western Blot
Reactivity:Mouse
Format:Lyophilized
See all Mouse IgG antibodies →
$317.00each
Catalog Number: LS-C745580-100

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Overview

Description
Mouse IgG antibody LS-C745580 is an Atto 647N-conjugated goat polyclonal antibody. Validated for FLISA, IF and WB.
Application
IF, Other, WB
Reactivity
Ms
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Secondary Antibodies
Recommended Dilution
ApplicationDilution
IF/ICC1:5000
OtherFLISA: 1:20000
WB1:10000

Target

Target
Mouse IgG
Antigen Species
Mouse
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins. This antibody will react with heavy chains of mouse IgG and with light chains of most mouse immunoglobulins.

Antibody

Host Species
Goat
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Mouse IgG whole molecule
Immunogen Type
Native Protein (Purified from Natural Source)

Format

Conjugate/Label
Atto 647N
Format
Lyophilized
Formulation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.