Goat anti Glucose Dehydrogenase antibody was used to detect purified Glucose Dehydrogenase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody. Detection shown was using Dylight 488 conjugated Donkey anti goat. Images were collected using the BioRad VersaDoc System

Glucose Dehydrogenase Antibody (Liquid, HRP)

Primary AntibodyLSBio Guarantee

Antibody:	Goat Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity:	Bacteria
Format:	Liquid

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$311.00each

Catalog Number: LS-C744726-25

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: Glucose Dehydrogenase antibody LS-C744726 is an HRP-conjugated goat polyclonal antibody to bacillus Glucose Dehydrogenase. Validated for ELISA and WB.
Application: ELISA, WB
Reactivity: Bac
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Recommended Dilution: ELISA: 1:10000 - 1:40000
Recommended Dilution: WB: 1:1000 - 1:5000

Target

Target: Bacillus Glucose Dehydrogenase
Antigen Species: Bacteria
Epitope: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Glucose Dehydrogenase [Bacillus]. Cross reactivity against Glucose Dehydrogenase from other sources is unknown.

Antibody

Host Species: Goat
Clonality: Polyclonal
Isotype: IgG

Immunogen

Immunogen: Glucose Dehydrogenase [Bacillus].

Format

Conjugate/Label: Horseradish Peroxidase
Format: Liquid
Formulation: 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
Concentration: 1 mg/mL
Volume: 25 Microliter
Preservative: Yes
Purification Method: Ion Exchange Chromatography

Storage & Handling

Storage Conditions: Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Recommended Storage Buffer: 0.02 M Potassium Phosphate|0.15 M NaCl