Menu
Products
Services
Research Areas
Resources
Contact Us
Distributors
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Services
Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
Contact Us
LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121
Phone:
866-819-4732 (Toll Free North America
206-374-1102 (International)
Fax:
866-206-6909 (Toll Free North America)
206-577-4565 (International)
How To Buy - Details about how to buy our products.
Orders@LSBio.com - To submit a new order.
Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, froforma invoice requests, or other billing issues.
Technical.Support@LSBio.com - To request technical information about an LSBio product or its applications
Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.
Worldwide Distributors List - To find your local distributor if you're not within the United States.
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-C154182-1 1 mg (1.1 mg/ml) $475 
GFP Antibody - Immunofluorescence Microscopy of Anti-GFP (GOAT) Antibody. Tissue: E5.5 Hex-GFP transgenic mouse embryo. Primary antibody: Goat anti-GFP was used at 1:500 dilution. Secondary antibody: Fluorchrome conjugated Anti-goat IgG secondary antibody at 1:10,000 for 45 min at RT. Staining: GFP as green fluorescent signal with DAPI blue counterstain.
GFP Antibody - Western Blot - Anti-GFP Antibody. Western blot of GFP recombinant protein detected with polyclonal anti-GFP antibody. Lane 1 shows detection of a 33 kD band corresponding to a GFP containing recombinant protein (arrowhead) expressed in HeLa cells. Lane 2 shows no staining of a mock transfected HeLa cell lysate. A 4-12% Bis-Tris gradient gel was used for SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody diluted to 1 ug/ml for 1 h at room temperature followed by washes and reaction with a 1:2500 dilution of IRDye 800 conjugated Donkey-a-Goat IgG [H&L] MX7 (. The IRDye 800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
GFP Antibody - Immunofluorescence Microscopy of Anti-GFP (GOAT) Antibody. Tissue: E5.5 Hex-GFP transgenic mouse embryo. Primary antibody: Goat anti-GFP was used at 1:500 dilution. Secondary antibody: Fluorchrome conjugated Anti-goat IgG secondary antibody at 1:10,000 for 45 min at RT. Staining: GFP as green fluorescent signal with DAPI blue counterstain.
GFP Antibody - Western Blot - Anti-GFP Antibody. Western blot of GFP recombinant protein detected with polyclonal anti-GFP antibody. Lane 1 shows detection of a 33 kD band corresponding to a GFP containing recombinant protein (arrowhead) expressed in HeLa cells. Lane 2 shows no staining of a mock transfected HeLa cell lysate. A 4-12% Bis-Tris gradient gel was used for SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody diluted to 1 ug/ml for 1 h at room temperature followed by washes and reaction with a 1:2500 dilution of IRDye 800 conjugated Donkey-a-Goat IgG [H&L] MX7 (. The IRDye 800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
1 of 2
2 of 2

GFP Antibody LS‑C154182

GFP Antibody LS‑C154182

Antibody:
Goat Polyclonal to Aequorea victoria GFP
Application:
IHC, IF, WB, IP, ELISA
Reactivity:
Aequorea victoria
Format:
Unconjugated, Unmodified
Price
Catalog Number
$475 
LS-C154182-1
1 mg
1.1 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Aequorea victoria GFP
Application:
IHC, IF, WB, IP, ELISA
Reactivity:
Aequorea victoria
Format:
Unconjugated, Unmodified

Specifications

Description
GFP antibody LS-C154182 is an unconjugated goat polyclonal antibody to aequorea victoria GFP. Validated for ELISA, IF, IHC, IP and WB.
Target
Aequorea victoria GFP
Host
Goat
Reactivity
Aequorea victoria (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC (1:200 - 1:1000)
  • Immunofluorescence (1:500)
  • Western blot (1:1000 - 1:5000)
  • Immunoprecipitation
  • ELISA (1:10000 - 1:30000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Specificity
Known to cross react with rGFP and eGFP. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria). No reaction was observed against Human, Mouse or Rat serum proteins.
Usage
Anti-GFP is designed to detect GFP and its variants. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with a monoclonal anti-GFP antibody using either form of the antibody as the capture or detection antibody. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin-HRP . Fluorochrome conjugated polyclonal anti-GFP can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E. coli) and eukaryotic (CHO cells) expression systems and detects GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP-containing proteins on western blots. Researchers should determine optimal titers for applications.
Presentation
Sterile Solution 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GFP
P42212 M62653 P42212

Publications (0)

Customer Reviews (0)


Requested From: United States
Date Requested: 11/17/2019