Human Apolipoprotein H Antibody (IgA) ELISA Kit (Sandwich ELISA)
Catalog Number: LS-F67373-1
1 plate / $591.00
Reactivity: Human
Detection Method Colorimetric
Format: 96-Well Strip Plate
Western Blot of Rat Anti-Nag 1 (C terminal specific). Lane 1: Protein Standard Opal Pre-stained at 1 µg/mL for overnight at 4°C. Secondary Antibody: Rat IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed at 1:40,000 dilution at RT for 30 minutes. Block: MB-070 at RT for 30 min.
GDF15 Antibody (C-Terminus, clone 6D12.H10.E4)
Primary AntibodyLSBio Guarantee
| Antibody: | Rat Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Liquid |
$311.00each
Catalog Number: LS-C745152-25
Catalog Number: LS-C745152
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- GDF15 antibody LS-C745152 is an unconjugated rat monoclonal antibody to GDF15 (C-Terminus) from human. It is reactive with human and mouse. Validated for ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Hu, Ms
- Predicted Reactivity
- Cyno, NHP
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- GDF15 | MIC1 | MIC-1 | NAG-1 | NRG-1 | NSAID-regulated gene 1 protein | PLAB | NSAID-activated gene 1 protein | Placental TGF-beta | PTGFB | GDF-15 | PTGF-beta
- Recommended Dilution: ELISA
- 1:2000
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- Q99988
- NCBI GenBank
- NM_004864, NP_004855.2
- SwissProt
- Q99988
Target
- Target
- Human GDF15
- Antigen Species
- Hu
- Epitope
- This antibody reacts with the C-terminus of endogenous NAG-1 protein from mouse tissues. A BLAST analysis suggests reactivity with NAG-1 from chimpanzee and macaque based on a 100% homology. Partial reactivity is expected against rat based on an 86% homology with the immunizing sequence. Cross-reactivity with NAG-1 from other sources has not been determined.
- Gene Name
- GDF15
Antibody
- Host Species
- Rt
- Clonality
- Monoclonal
- Clone
- 6D12.H10.E4
- Isotype
- IgG2a
- Light Chain
- λ
- Origin Species
- Hu
Immunogen
- Immunogen
- This Protein-A purified antibody was prepared by repeated immunizations with an MBP-tagged recombinant protein produced in E.coli corresponding to C-term mouse NAG-1 protein. Cross reactivity to MBP is negative.
- Immunogen Type
- Fusion-P
Format
- Conjugate/Label
- Unconj
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Volume
- 25 Microliter
- Preservative
- Yes
- Purification Method
- Prot G
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
