LSBio
Monoclonal Mouse anti-Human GATA4 Antibody (clone 10D8.B10.D4, aa1-100, WB)

Western Blot of Mouse anti-GATA4 Antibody. Lane 1: CCRF-CEM Lysate. Load: 25 µg per lane. Primary antibody: GATA-4 antibody at 1:500 overnight at 4°C. Secondary antibody: HRP mouse antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 44.5 kDa, 44.5 kDa for GATA4.

Monoclonal Mouse anti-Human GATA4 Antibody (clone 10D8.B10.D4, aa1-100, WB)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity:Human
Format:Liquid
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$311.00each
Catalog Number: LS-C744905-25

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Overview

Description
GATA4 antibody LS-C744905 is an unconjugated mouse monoclonal antibody to human GATA4 (aa1-100). Validated for ELISA and WB.
Application
ELISA, WB
Reactivity
Hu
Predicted Reactivity
Mouse, Rat
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
ASD2 | GATA-binding factor 4 | GATA-binding protein 4 | GATA4 | GATA binding protein 4 | Transcription factor GATA-4 | VSD1
UniProt Number
P43694
NCBI GenBank
NM_002052 | NP_002043.2
SwissProt
P43694
Recommended Dilution
ApplicationDilution
ELISA1:2000 - 1:10000
WB1:500 - 1:1000

Target

Target
Human GATA4
Antigen Species
Human
Epitope
A BLAST analysis was used to suggest reactivity with mouse and rat GATA4 based on a 93% homology. Cross-reactivity with GATA4 from other sources has not been determined.
Gene Name
GATA4

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
10D8.B10.D4
Isotype
IgG1
Light Chain
Kappa Light Chain
Origin Species
Human

Immunogen

Immunogen
GATA4 protein A purified antibody was prepared in mice by repeated immunizations with a recombinant protein corresponding to the first 100 amino acids of human GATA4 protein.
Immunogen Type
Recombinant Immunogen

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Volume
25 Microliter
Preservative
Yes
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.