LSBio
Polyclonal Rabbit anti-Mouse GATA1 Antibody (WB)

Western Blot of rabbit Anti-Gata1 antibody. Lane 1: NIH/3T3. Lane 2: mouse brain. Load: 25 µg per lane. Primary antibody: Gata1 antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:40,000 for 60 min at RT. Block: MB-070 Fluorescent Blocking Buffer used overnight at 4°C. Predicted/Observed size: 42.7 kDa for Gata1.

Polyclonal Rabbit anti-Mouse GATA1 Antibody (WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity:Mouse
Format:Liquid
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$311.00each
Catalog Number: LS-C745496-25

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Overview

Description
GATA1 antibody LS-C745496 is an unconjugated rabbit polyclonal antibody to mouse GATA1. Validated for ELISA and WB.
Application
ELISA, WB
Reactivity
Ms
Predicted Reactivity
Hu, Rt
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
ERYF1 | Globin transcription factor 1 | GATA-binding factor 1 | Erythroid transcription factor | GATA-1 | GF-1 | NFE1 | NF-E1 DNA-binding protein | Transcription factor GATA1 | XLTDA | XLTT | GATA1 | GF1 | XLANP
Recommended Dilution: ELISA
1:15000 - 1:75000
Recommended Dilution: WB
1:500 - 1:2000
UniProt Number
P15976
SwissProt
P15976

Target

Target
Mouse GATA1
Antigen Species
Ms
Epitope
A BLAST analysis was used to suggest reactivity with this protein from mouse and rat based on 100% homology for the immunogen sequence and 93% to human. Cross reactivity with Gata1 homologues from other sources has not been determined.
Gene Name
GATA1

Antibody

Host Species
Rb
Clonality
Polyclonal
Isotype
IgG
Origin Species
Hu

Immunogen

Immunogen
Anti-Gata1 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to mouse Gata1 peptide.
Immunogen Type
SP-Lin

Format

Conjugate/Label
Unconj
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
0.73 mg/mL
Volume
25 Microliter
Preservative
Yes
Purification Method
AP

Storage & Handling

Storage Conditions
Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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