Alpha SNAP Antibody
Catalog Number: LS-C401034
4 sizes / from $260.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Anti-FAP Antibody - Western Blot. Western blot of affinity purified anti-FAP antibody shows detection of FAP protein in whole cell lysates from FAP expressing HEK cells (lane 4) but not control HEK cells (lane 3). Specific band staining is blocked when the primary antibody is pre-incubated with immunizing peptide (lanes 1 and 2 respectively). The band at ~90 kD, indicated by the arrowhead, corresponds to the expected molecular weight of transfected FAP. Primary antibody was used at 1:1000. Personal communication, S. Kim, NCI, Bethesda, MD.
FAP Alpha Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Reactivity: | Mouse |
| Format: | Liquid |
$579.00each
Catalog Number: LS-C154234-100
Catalog Number: LS-C154234
For bulk orders or custom formulations:
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- Description
- FAP Alpha antibody LS-C154234 is an unconjugated rabbit polyclonal antibody to mouse FAP Alpha. Validated for ELISA and WB.
- Application
- ELISA, IHC, WB
- Reactivity
- Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- DPPIV | FAP alpha | FAP | Fibroblast activation protein | FAPA | Seprase
- UniProt Number
- Q12884
- NCBI GenBank
- NM_004460 | NP_004451.2
- SwissProt
- Q12884
Recommended Dilution
| Application | Dilution |
|---|---|
| ELISA | 1:10000 - 1:20000 |
| WB | 1:500 - 1:2000 |
Target
- Target
- Mouse FAP Alpha
- Antigen Species
- Mouse
- Epitope
- This antibody is known to react with mouse FAP protein. A BLAST analysis was used to suggest partial cross-reactivity with FAP from human and rat sources based on ~88% homology with the immunizing sequence. Reactivity with FAP from other sources has not been determined.
- Gene Name
- FAP
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse FAP protein.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1.1 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
