EPOR / EPO Receptor Antibody (Extracellular Domain)
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| Antibody: | Sheep Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunocytochemistry, Immunohistochemistry (general), Immunoprecipitation, Western Blot |
| Reactivity: | Human, Mouse |
| Format: | Liquid |
$461.00each
Catalog Number: LS-C84005-250
Catalog Number: LS-C84005
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- Description
- EPO Receptor antibody LS-C84005 is an unconjugated sheep polyclonal antibody to EPO Receptor (EPOR) (Extracellular Domain) from human. It is reactive with human and mouse. Validated for ELISA, ICC, IHC, IP and WB. Cited in 1 publication.
- Application
- ELISA, ICC, IHC, IP, WB
- Reactivity
- Hu, Ms
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- EPOR | EPO Receptor | EPO-R | Erythropoietin receptor
- Recommended Dilution: ELISA
- 10 ug/ml
- UniProt Number
- P19235
- NCBI GenBank
- NM_000121, NP_000112.1
- SwissProt
- P19235
Target
- Target
- Human EPOR / EPO Receptor
- Antigen Species
- Hu
- Epitope
- Antibody titers and specificity were analyzed by ELISA assay using control samples by varying concentration of GST-EpoR and control anti-GST antibody. Affinity purified antibody was found to detect EpoR at concentrations of 50 ng/ml. Neutralization of ligand binding has been detected using erythroleukemia cell line TF-1 with 3000 Epo receptors/cell to block Iodine 125 labeled erythropoietin ligand binding. This antibody will recognize EpoR-GST at its migration of 49 kD on SDS-PAGE. High quality secondary anti-sheep reagents are essential for this assay.
- Gene Name
- EPOR
Antibody
- Host Species
- Sh
- Clonality
- Polyclonal
- Origin Species
- Hu
Immunogen
- Immunogen
- Partial cDNA of the extracellular domain of human EpoR expressed as a GST fusion protein in E. coli.
- Immunogen Type
- Fusion-P
Format
- Conjugate/Label
- Unconj
- Format
- Liquid
- Formulation
- PBS, No preservatives added
- Concentration
- 2.68 mg/mL
- Preservative
- No
- Purification Method
- Prot G
Storage & Handling
- Storage Conditions
- Store at -20°C. Aliquot to avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS
Erythropoietin acts as an anti-inflammatory signal on murine mast cells. Wiedenmann T, Ehrhardt S, Cerny D, Hildebrand D, Klein S, Heeg K, Kubatzky KF. Molecular immunology. May;65:68-76. PubMed: 25645506