LSBio
EPCAM Antibody (clone HEA125)

EPCAM Antibody (clone HEA125)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin
Reactivity:Human
Format:Liquid
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$433.00each
Catalog Number: LS-C828457-0.1

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Overview

Description
EPCAM antibody LS-C828457 is an unconjugated mouse monoclonal antibody to human EPCAM. Validated for ELISA, Flow, IF and IHC.
Application
ELISA, FC, IF, IHC-F, IHC-P
Reactivity
Hu
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
323/A3 | ACSTD1 | 17-1A | CD326 | EGP | EGP34 | Epithelial glycoprotein | GA733-2 | HNPCC8 | Ep-CAM | ESA | HEGP314 | KS 1/4 antigen | KS1/4 | KSA | M1S2 | MIC18 | MK-1 | MH99 | TROP1 | TACST-1 | TACSTD1 | CD326 antigen | CO-17A | DIAR5 | EGP-2 | EGP314 | EGP40 | EPCAM | Epithelial glycoprotein 314 | HEA125 | Ly74 | M4S1
Recommended Dilution: IHC-F
1:100 - 1:500
Recommended Dilution: IHC-P
1:100 - 1:500
UniProt Number
P16422
SwissProt
P16422

Target

Target
Human EPCAM
Antigen Species
Hu
Epitope
The antibody HEA125 is directed against human EpCAM. he monoclonal antibody HEA125 shows a broad reactivity pattern with most human epithelial cells and epithelium derived tumours. It doesn't react with epidermal keratinocytes, parietal cells, hepatocytes, thymus cortical epithelium, myoepithelia, and non-epithelial tissues. HEA125 reacts intensely with all carcinomas derived from the intestinal tract, stomach, pancreas, liver, lung, breast, ovary, thyroid gland, kidney, bladder, prostate and metastases thereof. Stratified squamous epithelia usually stain less intensely then adenocarcinoma. The antibody is suitable for differentiation between carcinoma and non-carcinoma. Keratinizing areas of a tumour mass usually remain unstained as sarcoma, lymphoma, melanoma and neurogenic tumours do.
Gene Name
EPCAM

Antibody

Host Species
Ms
Clonality
Monoclonal
Clone
HEA125
Isotype
IgG1
Origin Species
Hu

Immunogen

Immunogen
Viable HT-29 human colon carcinoma cell line
Immunogen Type
Cell

Format

Conjugate/Label
Unconj
Format
Liquid
Formulation
PBS, 0.09% Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Other

Storage & Handling

Storage Conditions
Store at 4°C for short term storage. Aliquot and store at -20°C for long term storage. Avoid freeze/thaw cycles. Prior to use, thaw aliquot slowly in the dark at ambient temperature. Spin down and prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Store working dilutions at 4°C, and preferably use the same day. Remove slight precipitation with centrifugation.
Recommended Storage Buffer
PBS

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.