Human Apolipoprotein H Antibody (IgA) ELISA Kit (Sandwich ELISA)
Catalog Number: LS-F67373-1
1 plate / $591.00
Reactivity: Human
Detection Method Colorimetric
Format: 96-Well Strip Plate
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Dityrosine Monoclonal Antibody, Clone 7D4. Tissue: Embryonic kidney cells (HEK293) cultured overnight with 50 µM H2O2. Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Dityrosine Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Localization: Cytoplasmic. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alexa Fluor 633 F-Actin stain. (C) Dityrosine Antibody. (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
Dityrosine Antibody (clone 7D4, APC)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Western Blot |
| Reactivity: | Pan-species / Cross-reactive |
| Format: | Liquid |
$544.00each
Catalog Number: LS-C821090-100
Catalog Number: LS-C821090
For bulk orders or custom formulations:
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- Description
- Dityrosine antibody LS-C821090 is an APC-conjugated mouse monoclonal antibody to all species Dityrosine. Validated for ELISA, IF and WB.
- Application
- ELISA, IF, WB
- Reactivity
- Pan
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Recommended Dilution: ELISA
- 1:1000
- Recommended Dilution: IF/ICC
- 1:50
- Recommended Dilution: WB
- 1:1000
Target
- Target
- All species Dityrosine
- Antigen Species
- Pan
- Epitope
- Specific for dityrosine modified proteins. Does not cross-react with 3,5-dibromotyrosine or bromotyrosine modified proteins.
Antibody
- Host Species
- Ms
- Clonality
- Monoclonal
- Clone
- 7D4
- Isotype
- IgG1
Immunogen
- Immunogen
- Synthetic Dityrosine conjugated to Keyhole Limpet Hemocyanin (KLH).
- Immunogen Type
- Synth
Format
- Conjugate/Label
- APC
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Prot G
Storage & Handling
- Storage Conditions
- Store at -20°C.
- Recommended Storage Buffer
- PBS
