Flow Cytometry analysis of K562 cells using anti-Cytochrome C antibody. Overlay histogram showing K562 cells stained with anti-Cytochrome C antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Cytochrome C Antibody (µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
CYCS / Cytochrome c Antibody (clone 15F10)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Lyophilized |
$480.00each
Catalog Number: LS-C756672-100
Catalog Number: LS-C756672
For bulk orders or custom formulations:
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- Description
- Cytochrome c antibody LS-C756672 is an unconjugated mouse monoclonal antibody to Cytochrome c (CYCS) from human. It is reactive with human, mouse and rat. Validated for Flow, ICC, IHC and WB.
- Application
- FC, ICC, IHC-F, IHC-P, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- CYC | HCS | THC4 | CYCS | Cytochrome c | Cytochrome c, somatic
- Recommended Dilution: FC
- 1 - 3 µg/10E6 cells
- Recommended Dilution: IF/ICC
- 0.5 - 1 µg/ml
- Recommended Dilution: IHC-F
- 0.5 - 1 µg/ml
- Recommended Dilution: IHC-P
- 0.5 - 1 µg/ml
- Recommended Dilution: WB
- 0.1 - 0.5 µg/ml
- UniProt Number
- P99999
- NCBI GenBank
- NM_018947, NP_061820.1
- SwissProt
- P99999
Target
- Target
- Human CYCS / Cytochrome c
- Antigen Species
- Hu
- Epitope
- No cross reactivity with other proteins.
- Gene Name
- CYCS
Antibody
- Host Species
- Ms
- Clonality
- Monoclonal
- Clone
- 15F10
- Isotype
- IgG1
- Origin Species
- Hu
Immunogen
- Immunogen
- E.coli-derived human Cytochrome C recombinant protein (Position: G2-E105). Human Cytochrome C shares 91% amino acid (aa) sequence identity with both mouse and rat Cytochrome C.
- Immunogen Type
- Recom
Format
- Conjugate/Label
- Unconj
- Format
- Lyophilized
- Formulation
- Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
- Preservative
- Yes
- Purification Method
- AP
Storage & Handling
- Storage Conditions
- After reconstitution, may be stored at 4°C for 1 month. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C.
- Recommended Storage Buffer
- NaCl