Human Apolipoprotein H Antibody (IgA) ELISA Kit (Sandwich ELISA)
Catalog Number: LS-F67373-1
1 plate / $591.00
Reactivity: Human
Detection Method Colorimetric
Format: 96-Well Strip Plate
ELISA of results purified Sheep anti-Cyanine Antibody. Each well was coated in duplicate with 1.0 µg of BSA-conjugated Cy5. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using blocking buffer MB-Donkey anti-Sheep HRP conjugate 1:10,000, and TMB-8000 substrate.
Cyanine Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Sheep Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunoprecipitation, Other, Western Blot |
| Format: | Liquid |
$619.00each
Catalog Number: LS-C154306-1
Catalog Number: LS-C154306
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- Cyanine antibody LS-C154306 is an unconjugated sheep polyclonal antibody to Cyanine. Validated for ELISA, IHC and WB.
- Application
- ELISA, IHC, IP, Other, WB
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Recommended Dilution: ELISA
- 1:10000 - 1:50000
- Recommended Dilution: WB
- 1:1000 - 1:5000
Target
- Target
- Cyanine
- Epitope
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum and cyanine conjugated IgG. Reactivity was observed against Cy2, Cy3 and Cy5. Reactivity against other cyanine analogs is unknown.
Antibody
- Host Species
- Sh
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- A mixture of Cy3 and Cy5 conjugated KLH.
Format
- Conjugate/Label
- Unconj
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1.1 mg/mL
- Preservative
- Yes
- Purification Method
- AP
Storage & Handling
- Storage Conditions
- Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
