Western blot using the protein-A purified anti-bovine CXCL10 antibody shows detection of recombinant bovine CXCL10 at 9.3 kDa (arrowhead) raised in yeast. The protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with 1 µg/mL primary antibody overnight at 4°C. After washing, membrane was probed with for 45 min at room temperature.
CXCL10 / IP-10 Antibody (Liquid)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Bovine |
| Format: | Liquid |
$311.00each
Catalog Number: LS-C745206-25
Catalog Number: LS-C745206
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- Description
- IP-10 antibody LS-C745206 is an unconjugated rabbit polyclonal antibody to IP-10 (CXCL10) from bovine. It is reactive with bovine, goat and sheep. Validated for ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Bov
- Predicted Reactivity
- Goat, Sheep
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- C-X-C motif chemokine 10 | CXCL10 | Gamma-IP10 | IP-10 | Gamma IP10 | GIP-10 | Mob-1 | Small-inducible cytokine B10 | Crg-2 | IFI10 | INP10 | SCYB10
- UniProt Number
- P02778
- NCBI GenBank
- NM_001565 | NP_001556.2
- SwissProt
- P02778
Recommended Dilution
| Application | Dilution |
|---|---|
| ELISA | 1:10000 |
| WB | 1 µg/ml |
Target
- Target
- Bovine CXCL10 / IP-10
- Antigen Species
- Bovine
- Epitope
- This antibody is specific for bovine CXCL10 protein. A BLAST analysis was used to suggest cross-reactivity with CXCL10 from bovine based on 100% homology with the immunizing sequence. Partial reactivity is expected against sheep and goat based on 94-95% homology. Cross-reactivity with CXCL10 from other sources has not been determined.
- Gene Name
- CXCL10
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to the 83 amino acids of the mature bovine CXCL10 protein.
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1.17 mg/mL
- Volume
- 25 Microliter
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution for 3 months. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl