Western blot of Biotin conjugated Rabbit Anti-Fractalkine primary antibody. Lane 1: Human Fractalkine. Lane 2: None. Load: 50 ng per lane. Primary antibody: Fractalkine Biotin conjugated antibody at 1:1,000 for 60 min at RT. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:40,000 for 30 min at RT. Blocking: MB-070 for 30 min at RT. Observed/Predicted size: 9 kDa, 9 kDa for Human Fractalkine. Other band(s): None. This image was taken for the unconjugated form of this product. Other forms have not been tested.
CX3CL1 / Fractalkine Antibody (Lyophilized, Biotin)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Human |
| Format: | Lyophilized |
$564.00each
Catalog Number: LS-C209280-100
Catalog Number: LS-C209280
For bulk orders or custom formulations:
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- Description
- Fractalkine antibody LS-C209280 is a biotin-conjugated rabbit polyclonal antibody to human Fractalkine (CX3CL1). Validated for ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- ABCD-3 | C-X3-C motif chemokine 1 | C3Xkine | Chemokine ntt | CX3CL1 | CXC3C | FKN | Fractalkine | Neurotactin | CXC3 | NTN | SCYD1 | Small-inducible cytokine D1
- Recommended Dilution: ELISA
- 1:20000 - 1:50000
- Recommended Dilution: WB
- 1:1000 - 1:2000
- UniProt Number
- P78423
- NCBI GenBank
- NM_002996 | NP_002987.1
- SwissProt
- P78423
Target
- Target
- Human CX3CL1 / Fractalkine
- Antigen Species
- Human
- Epitope
- This antibody is specific for human Fractalkine protein. A BLAST analysis was used to suggest cross-reactivity with Fractalkine from human sources based on 100% homology with the immunizing sequence. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin and anti-Rabbit Serum. Cross-reactivity with Fractalkine from other sources has not been determined.
- Gene Name
- CX3CL1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Anti-Fractalkine antibody was prepared from rabbit antiserum after repeated immunizations with a truncated form of recombinant human Fractalkine protein from E. coli.
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Biotin
- Format
- Lyophilized
- Formulation
- Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Ion Exchange Chromatography
Storage & Handling
- Storage Conditions
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl