LSBio
Monoclonal Mouse anti-Human CTLA4 / CD152 Antibody (clone BNI3, IHC, IF)

Monoclonal Mouse anti-Human CTLA4 / CD152 Antibody (clone BNI3, IHC, IF)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Flow Cytometry, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunoprecipitation
Reactivity:Human
Format:Liquid
See all Human CTLA4 / CD152 antibodies →
$534.00each
Catalog Number: LS-C43849-0.2

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Overview

Description
CD152 antibody LS-C43849 is an unconjugated mouse monoclonal antibody to human CD152 (CTLA4). Validated for Flow, IF, IHC and IP.
Application
FC, IF, IHC, IHC-F, IP
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
CD152 | CELIAC3 | CD152 antigen | CTLA-4 | CTLA4 | Celiac disease 3 | IDDM12 | GRD4 | GSE | CD152 isoform
Recommended Dilution: FC
1:1 - 1:10
UniProt Number
P16410
NCBI GenBank
NM_005214 | NP_005205.2
SwissProt
P16410

Target

Target
Human CTLA4 / CD152
Antigen Species
Human
Epitope
Recognizes human CD152, also known as CTLA-4 (cytotoxic T-lymphocyte-associated antigen 4), an inhibitory receptor and negative regulator of T-cell responses. CD152 is a single pass type 1 transmembrane protein belonging to the immunoglobulin superfamily containing a single domain in the extracellular region. CD152 along with CD28 binds to the co-stimulatory molecules CD80 and CD86. Is able to block ligand binding on the Raji B-cell line and blocks binding of an alternative clone, BNI8 to CTLA-4/Ig in ELISA. Further studies indicate that clone BNI3 binds to the same epitope as classified anti CTLA-4 clones 11D4 and 10A8 (Wang et al. In: Leukocyte typing VI 1997 Garland Publishing Inc. pp97-98,. The cytoplasmic domain of CD152 contains a critical tyrosine at residue 201 phosphorylated by Janus Kinase 2 which subsequently controls surface expression through regulation of CD152 interaction with AP-2. CD152 is expressed primarily as an intracellular antigen with transport to the cell surface under tight regulation of several molecules including Trim, PLD and TIRC7, CD152 also demonstrates rapid internalization once expressed at the cell surface. CD152 plays a significant role in maintaining tolerance to self antigens and defects in CD152 presentation and expression has been implicated in a number of autoimmune diseases.
Gene Name
CTLA4

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
BNI3
Isotype
IgG2a

Immunogen

Immunogen
Human CTLA-4/human IgG heavy chain fusion protein.
Immunogen Type
Fusion Protein (e.g., GST, MBP tag)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, 0.09% Sodium Azide, 0.5% BSA
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Recommended Storage Buffer
PBS

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.