LSBio
Polyclonal Rabbit anti-Mammal Collagen IV Antibody (FITC, IHC, WB)

Polyclonal Rabbit anti-Mammal Collagen IV Antibody (FITC, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunoprecipitation, Western Blot
Reactivity:Bovine, Human, Mammal (Other)
Format:Lyophilized
See all Mammal Collagen IV antibodies →
$594.00each
Catalog Number: LS-C745505-50

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Overview

Description
Collagen IV antibody LS-C745505 is an FITC-conjugated rabbit polyclonal antibody to Collagen IV from mammal. It is reactive with human, bovine and mammal. Validated for ELISA, IHC, IP and WB.
Application
ELISA, IHC, IP, WB
Reactivity
Bov, Hu, Mml
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Recommended Dilution: ELISA
1:20000 - 1:100000
Recommended Dilution: IHC
1:1000 - 1:5000
Recommended Dilution: IP
1:100
Recommended Dilution: WB
1:2000 - 1:10000

Target

Target
Mammal Collagen IV
Antigen Species
Mml
Epitope
Typically negligible cross-reactivity against other types of collagens was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type IV collagens and has negligible cross-reactivity with Type I, II, III, V and VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

Antibody

Host Species
Rb
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Anti-Collagen Type IV was produced by repeated immunizations with Collagen Type IV from human and bovine placenta.
Immunogen Type
Native

Format

Conjugate/Label
FITC
Format
Lyophilized
Formulation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.01 M Sodium Phosphate, pH 7.2, 0.25 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
AP

Storage & Handling

Storage Conditions
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Recommended Storage Buffer
0.01 M Sodium Phosphate|0.25 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.