Collagen IV Antibody (clone C IV 22)
Catalog Number: LS-C96275-100
100 µg / $847.00
Antibody: Mouse Monoclonal
Applications: Immunohistochemistry (general), Immunohistochemistry, Paraffin
Reactivity: Human
Format: Lyophilized
Anti collagen IV antibody showed strong staining in FFPE sections of human kidney (Left) with strong red staining observed in glomeruli; and liver (Right) with strong staining in sinusoids. Staining for both tissues was consistent with a basement membrane distribution. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval.
Collagen IV Antibody
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunoprecipitation, Western Blot |
| Reactivity: | Bovine, Human, Mammal (Other) |
| Format: | Liquid |
$311.00each
Catalog Number: LS-C745278-25
Catalog Number: LS-C745278
For bulk orders or custom formulations:
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- Description
- Collagen IV antibody LS-C745278 is an unconjugated rabbit polyclonal antibody to Collagen IV from mammal. It is reactive with human, bovine and mammal. Validated for ELISA, IHC, IP and WB.
- Application
- ELISA, IHC, IP, WB
- Reactivity
- Bov, Hu, Mml
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Recommended Dilution: ELISA
- 1:5000 - 1:50000
- Recommended Dilution: IHC
- 1:50 - 1:200
- Recommended Dilution: IP
- 1:100
- Recommended Dilution: WB
- 1:1000 - 1:10000
Target
- Target
- Mammal Collagen IV
- Antigen Species
- Mml
- Epitope
- Typically negligible cross-reactivity against other types of collagens was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type IV collagens and has negligible cross-reactivity with Type I, II, III, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Antibody
- Host Species
- Rb
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Collagen Type IV from human and bovine placenta.
- Immunogen Type
- Native
Format
- Conjugate/Label
- Unconj
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Volume
- 25 Microliter
- Preservative
- Yes
- Purification Method
- AP
Storage & Handling
- Storage Conditions
- Store vial at 4°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
