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Catalog Number Size Price
LS-C745076-25 25 µl $304 
CELA1 / Pancreatic Elastase 1 Antibody - Double immunofluorescence microscopy of rabbit anti-Elastase antibody and anti-Insulin antibody. Tissue: mouse embryonic gut tissue. Fixation: 1 h in 4% PFA. Antigen retrieval: not required. Primary antibody: antibodies at 10 µg/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: elastase is secreted, extracellular. Staining: elastase as green fluorescent signal with insulin staining in red.
CELA1 / Pancreatic Elastase 1 Antibody - Western Blot of rabbit anti-Elastase Antibody. Lane 1: Elastase. Load: 50 ng per lane. Primary antibody: Rabbit anti-Elastase Antibody at 1:1,000 overnight at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:40,000 for 30 minutes at RT. Block: MB-070 for 30 minutes at RT. Predicted/Observed size: 29 kDa, 29 kDa for Elastase.
CELA1 / Pancreatic Elastase 1 Antibody - Double immunofluorescence microscopy of rabbit anti-Elastase antibody and anti-Insulin antibody. Tissue: mouse embryonic gut tissue. Fixation: 1 h in 4% PFA. Antigen retrieval: not required. Primary antibody: antibodies at 10 µg/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: elastase is secreted, extracellular. Staining: elastase as green fluorescent signal with insulin staining in red.
CELA1 / Pancreatic Elastase 1 Antibody - Western Blot of rabbit anti-Elastase Antibody. Lane 1: Elastase. Load: 50 ng per lane. Primary antibody: Rabbit anti-Elastase Antibody at 1:1,000 overnight at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:40,000 for 30 minutes at RT. Block: MB-070 for 30 minutes at RT. Predicted/Observed size: 29 kDa, 29 kDa for Elastase.
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Polyclonal Rabbit anti‑Pig CELA1 / Pancreatic Elastase 1 Antibody (Liquid, IF, WB) LS‑C745076

Polyclonal Rabbit anti‑Pig CELA1 / Pancreatic Elastase 1 Antibody (Liquid, IF, WB) LS‑C745076

Antibody:
CELA1 / Pancreatic Elastase 1 Rabbit anti-Pig Polyclonal (Liquid) Antibody
Application:
IF, WB, ELISA
Reactivity:
Pig
Format:
Unconjugated, Liquid
Price
Catalog Number
$304
LS-C745076-25
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CELA1 / Pancreatic Elastase 1 Rabbit anti-Pig Polyclonal (Liquid) Antibody
Application:
IF, WB, ELISA
Reactivity:
Pig
Format:
Unconjugated, Liquid

Specifications

Description
Pancreatic Elastase 1 antibody LS-C745076 is an unconjugated rabbit polyclonal antibody to pig Pancreatic Elastase 1 (CELA1). Validated for ELISA, IF and WB.
Target
Pig CELA1 / Pancreatic Elastase 1
Synonyms
CELA1 | El1a | Elastase I | ELA1 | Elastase 1, pancreatic | Elastase-1 | Pancreatic elastase | Pancreatic elastase I | Porcine pancreatic elastase | Pancreatic elastase 1 | Elastase
Host
Rabbit
Reactivity
Pig (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Liquid
Immunogen
Elastase [Porcine Pancreas].
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Elastase [Porcine Pancreas]. Cross reactivity was reported for mouse Elastase (mouse embryonic pancreas). Cross reactivity against Elastase from other tissues and species has not been determined.
Applications
  • Immunofluorescence
  • Western blot (1:500 - 1:3000)
  • ELISA (1:5000 - 1:25000)
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CELA1 / Pancreatic Elastase 1
Q9UNI1 NM_001971 NP_001962.3

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Requested From: United States
Date Requested: 4/29/2024