LSBio
Polyclonal Rabbit anti-Pig CELA1 / Pancreatic Elastase 1 Antibody (Liquid, IF, WB)

Double immunofluorescence microscopy of rabbit anti-Elastase antibody and anti-Insulin antibody. Tissue: mouse embryonic gut tissue. Fixation: 1 h in 4% PFA. Antigen retrieval: not required. Primary antibody: antibodies at 10 µg/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: elastase is secreted, extracellular. Staining: elastase as green fluorescent signal with insulin staining in red.

Polyclonal Rabbit anti-Pig CELA1 / Pancreatic Elastase 1 Antibody (Liquid, IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Western Blot
Reactivity:Pig
Format:Liquid
See all Pig CELA1 / Pancreatic Elastase 1 antibodies →
$311.00each
Catalog Number: LS-C745076-25

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Overview

Description
Pancreatic Elastase 1 antibody LS-C745076 is an unconjugated rabbit polyclonal antibody to pig Pancreatic Elastase 1 (CELA1). Validated for ELISA, IF and WB.
Application
ELISA, IF, WB
Reactivity
Por
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
CELA1 | El1a | Elastase I | ELA1 | Elastase 1, pancreatic | Elastase-1 | Pancreatic elastase | Pancreatic elastase I | Porcine pancreatic elastase | Pancreatic elastase 1 | Elastase
Recommended Dilution: ELISA
1:5000 - 1:25000
Recommended Dilution: WB
1:500 - 1:3000
UniProt Number
Q9UNI1
SwissProt
Q9UNI1

Target

Target
Pig CELA1 / Pancreatic Elastase 1
Antigen Species
Pig
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Elastase [Porcine Pancreas]. Cross reactivity was reported for mouse Elastase (mouse embryonic pancreas). Cross reactivity against Elastase from other tissues and species has not been determined.
Gene Name
CELA1

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Origin Species
Human

Immunogen

Immunogen
Elastase [Porcine Pancreas].

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Volume
25 Microliter
Preservative
Yes
Purification Method
Ion Exchange Chromatography

Storage & Handling

Storage Conditions
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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