Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CD74 Monoclonal Antibody, Clone 6D9. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-CD74 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell membrane, Endoplasmic Reticulum, Golgi apparatus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) CD74 Antibody. (D) Composite.
CD74 / CLIP Antibody (clone 6D9, APC)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Immunofluorescence, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$488.00each
Catalog Number: LS-C776005-100
Catalog Number: LS-C776005
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- Description
- CLIP antibody LS-C776005 is an APC-conjugated mouse monoclonal antibody to human CLIP (CD74). Validated for IF and WB.
- Application
- IF, WB
- Reactivity
- Hu
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- CD74 antigen | CD74 | DHLAG | HLADG | HLA-DR-gamma | Ia-associated invariant chain | p33 | MHC HLA-DR gamma chain | Ia-GAMMA
- Recommended Dilution: IF/ICC
- 1:100
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- P04233
- NCBI GenBank
- NM_004355, NP_004346.1
- SwissProt
- P04233
Target
- Target
- Human CD74 / CLIP
- Antigen Species
- Hu
- Epitope
- Detects ~33 kDa protein.
- Gene Name
- CD74
Antibody
- Host Species
- Ms
- Clonality
- Monoclonal
- Clone
- 6D9
- Isotype
- IgG1
- Origin Species
- Hu
Immunogen
- Immunogen
- Synthetic peptide corresponding to the N-Terminus (1-100 aa) of human CD74 conjugated to KLH.
- Immunogen Type
- SP-Lin
Format
- Conjugate/Label
- APC
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Prot G
Storage & Handling
- Storage Conditions
- Store at -20°C.
- Recommended Storage Buffer
- PBS