LSBio
CD3E Antibody (clone APA1/1)

Intracellular staining of human peripheral blood with anti-CD3 epsilon activation epitope (APA1/1) purified, GAM-FITC.

CD3E Antibody (clone APA1/1)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Flow Cytometry, Immunocytochemistry, Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunoprecipitation, Western Blot
Reactivity:Human, Mouse
Format:Liquid
See all Human CD3E antibodies →
$323.00each
Catalog Number: LS-C112447-0.1

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Overview

Description
CD3E antibody LS-C112447 is an unconjugated mouse monoclonal antibody to CD3E from human. It is reactive with human and mouse. Validated for Flow, ICC, IHC, IP and WB.
Application
FC, ICC, IHC, IHC-F, IP, WB
Reactivity
Hu, Ms
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
CD3e antigen | CD3E | CD3-epsilon | T-cell receptor epsilon chain | TCRE | T-cell receptors epsilon chain | T3E | CD3 Epsilon | CD3

Target

Target
Human CD3E
Antigen Species
Hu
Epitope
The mouse monoclonal antibody APA1/1 recognizes an activation-dependent intracellular epitope of CD3 epsilon. Exposure of the epitope precedes CD3 phosphorylation and recruitment and activation of ZAP70, which initiates the signaling cascade produced by T-cell activation. APA1/1 provides the earliest known marker for TCR-mediated T cell activation.

Antibody

Host Species
Ms
Clonality
Monoclonal
Clone
APA1/1
Isotype
IgG1

Immunogen

Immunogen
Purified human CD3 proteins isolated from thymus.
Immunogen Type
Native

Format

Conjugate/Label
Unconj
Format
Liquid
Formulation
PBS, pH 7.4, 15 mM Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Prot A

Storage & Handling

Storage Conditions
Store at 2°C to 8°C. Do not freeze.
Recommended Storage Buffer
PBS

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