Monoclonal Rat anti-Mouse CD90.2 (Mouse) Antibody (clone 30H12, AF647)
Catalog Number: LS-C810655
2 sizes / from $259.00
Antibody: Rat Monoclonal
Applications: Flow Cytometry
Reactivity: Mouse
Format: Liquid
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD274 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD274.
Monoclonal Mouse anti-Human CD274 / B7-H1 / PD-L1 Antibody (clone OTI2C7, IF, WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$443.00each
Catalog Number: LS-C338364-100
Catalog Number: LS-C338364
For bulk orders or custom formulations:
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- Description
- PD-L1 antibody LS-C338364 is an unconjugated mouse monoclonal antibody to human PD-L1 (CD274 / B7-H1). Validated for IF and WB. Cited in 3 publications.
- Application
- IF, IHC, WB
- Reactivity
- Hu
- Nonreactive Species
- Mouse
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- B7-H | B7 homolog 1 | B7-H1 | B7H1 | CD274 molecule | CD274 | CD274 antigen | PDCD1 ligand 1 | PDL1 | PD-L1 | PDCD1L1 | PDCD1LG1 | Programmed death ligand 1
- Recommended Dilution: IF/ICC
- 1:100
- Recommended Dilution: WB
- 1:200 - 1:2000
- UniProt Number
- Q9NZQ7
- NCBI GenBank
- NM_014143 | NP_054862.1
- SwissProt
- Q9NZQ7
Target
- Target
- Human CD274 / B7-H1 / PD-L1
- Antigen Species
- Human
- Epitope
- Human B7-H1 / PD-L1 / CD274
- Gene Name
- CD274
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- OTI2C7
- Isotype
- IgG1
Immunogen
- Immunogen
- Full length human recombinant protein of human CD274(NP_054862) produced in HEK293T cell.
- Immunogen Type
- Full-Length Recombinant Protein
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
- Concentration
- 1 mg/mL
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS
Juxtacrine Signaling Inhibits Antitumor Immunity by Upregulating PD-L1 Expression. Wen-Hao Yang, Jong-Ho Cha, Weiya Xia, Heng-Huan Lee, Li-Chuan Chan, Ying-Nai Wang, Jennifer L Hsu, Guoxin Ren, Mien-Chie Hung. Cancer research. 2018 July;78:3761-3768.
PubMed: 29789418 · PMC: PMC6050079
PD-L1-mediated gasdermin C expression switches apoptosis to pyroptosis in cancer cells and facilitates tumour necrosis. Junwei Hou, Rongce Zhao, Weiya Xia, Chiung-Wen Chang, Yun You, Jung-Mao Hsu, Lei Nie, Yeh Chen, Yu-Chuan Wang, Chunxiao Liu, Wei-Jan Wang, Yun Wu, Baozhen Ke, Jennifer L Hsu, Kebin Huang, Zu Ye, Yi Yang, Xianghou Xia, Yintao Li, Chia-Wei Li, Bin Shao, John A Tainer, Mien-Chie Hung. Nature cell biology. 2020 October;22:1264-1275.
PubMed: 32929201 · PMC: PMC7653546
Metformin Promotes Antitumor Immunity via Endoplasmic-Reticulum-Associated Degradation of PD-L110<sup> Department of Experimental Radiation Oncology, University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA12<sup> Department of Breast Surgical Oncology, University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA14<sup> Department of Experimental Radiation Oncology, University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA; Graduate School of Biomedical Sciences. Jong-Ho Cha, Wen-Hao Yang, Weiya Xia, Yongkun Wei, Li-Chuan Chan, Seung-Oe Lim, Chia-Wei Li, Taewan Kim, Shih-Shin Chang, Heng-Huan Lee, Jennifer L Hsu, Hung-Ling Wang, Chu-Wei Kuo, Wei-Chao Chang, Sirwan Hadad, Colin A Purdie, Aaron M McCoy, Shirong Cai, Yizheng Tu, Jennifer K Litton, Elizabeth A Mittendorf, Stacy L Moulder, William F Symmans, Alastair M Thompson, Helen Piwnica-Worms, Chung-Hsuan Chen, Kay-Hooi Khoo, Mien-Chie Hung. Molecular cell. 2018 Aug;71:606-620.e7.
PubMed: 30118680 · PMC: PMC6786495
