Fig-1: Cell Surface flow analysis of PD-L1 in 3 day-PHA treated human PBMC cells using 1 µg/10^6 cells of PD-L1 antibody. Green represents isotype control; red represents anti-PD-L1 antibody. Goat anti-mouse PE conjugate was used as secondary antibody.
CD274 / B7-H1 / PD-L1 Antibody (Azide-free)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Flow Cytometry, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$416.00each
Catalog Number: LS-C783213-100
Catalog Number: LS-C783213
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- Description
- PD-L1 antibody LS-C783213 is an unconjugated mouse monoclonal antibody to human PD-L1 (CD274 / B7-H1). Validated for Flow, IHC and WB.
- Application
- FC, IHC, WB
- Reactivity
- Hu
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- B7-H | B7 homolog 1 | B7-H1 | B7H1 | CD274 molecule | CD274 | CD274 antigen | PDCD1 ligand 1 | PDL1 | PD-L1 | PDCD1L1 | PDCD1LG1 | Programmed death ligand 1
- Recommended Dilution: FC
- 0.5 - 1 µg/10E6 cells
- Recommended Dilution: IHC
- 5 - 10 µg/ml
- Recommended Dilution: WB
- 2 - 4 µg/ml
- UniProt Number
- Q9NZQ7
- NCBI GenBank
- NM_014143, NP_054862.1
- SwissProt
- Q9NZQ7
Target
- Target
- Human CD274 / B7-H1 / PD-L1
- Antigen Species
- Hu
- Gene Name
- CD274
Antibody
- Host Species
- Ms
- Clonality
- Monoclonal
- Isotype
- IgG2b
- Light Chain
- κ
- Origin Species
- Hu
Immunogen
- Immunogen
- A partial length recombinant protein of PD-L1 (aa 13-224)
- Immunogen Type
- Frag-RP
Format
- Conjugate/Label
- Unconj
- Format
- Liquid
- Formulation
- PBS, 0.05% BSA
- Concentration
- 0.5 mg/mL
- Preservative
- No
- Purification Method
- Prot G
Storage & Handling
- Storage Conditions
- Store at 4°C for up to 6 months. For long-term storage, store at -20°C. Avoid freeze/thaw cycles.
- Recommended Storage Buffer
- PBS