LSBio
Polyclonal Rabbit anti-Human CA2 / Carbonic Anhydrase II Antibody (Liquid, HRP, IHC, WB)

Western Blot of rabbit anti-Carbonic Anhydrase II Antibody Peroxidase Conjugated. Lane 1: Carbonic Anhydrase II. Load: 50 ng per lane. Primary antibody: Rabbit anti-Carbonic Anhydrase II Antibody Peroxidase Conjugated at 1:1,000 overnight at 4°C. Secondary antibody: n/a. Block: MB-070 for 30 minutes at RT. Predicted/Observed size: 29 kDa, 29 kDa for Carbonic Anhydrase II.

Polyclonal Rabbit anti-Human CA2 / Carbonic Anhydrase II Antibody (Liquid, HRP, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:Human
Format:Liquid
See all Human CA2 / Carbonic Anhydrase II antibodies →
$311.00each
Catalog Number: LS-C745028-25

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Overview

Description
Carbonic Anhydrase II antibody LS-C745028 is an HRP-conjugated rabbit polyclonal antibody to human Carbonic Anhydrase II (CA2). Validated for ELISA, IHC and WB.
Application
ELISA, IHC, WB
Reactivity
Hu
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
CA-II | CAII | CAC | Car2 | Carbonate dehydratase II | Carbonic anhydrase C | Carbonic anhydrase II | Carbonic dehydratase | CA2 | Carbonic anhydrase 2 | Carbonic anhydrase B
Recommended Dilution: ELISA
1:500 - 1:2500
Recommended Dilution: IHC
1:100 - 1:500
Recommended Dilution: WB
1:250 - 1:1000
UniProt Number
P00918
SwissProt
P00918

Target

Target
Human CA2 / Carbonic Anhydrase II
Antigen Species
Hu
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Carbonic Anhydrase II [Human Erythrocytes]. Cross reactivity against Carbonic Anhydrase II from other sources may occur but have not been specifically determined.
Gene Name
CA2

Antibody

Host Species
Rb
Clonality
Polyclonal
Isotype
IgG
Origin Species
Hu

Immunogen

Immunogen
Carbonic Anhydrase II [Human Erythrocytes].

Format

Conjugate/Label
HRP
Format
Liquid
Formulation
10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
Concentration
1 mg/mL
Volume
25 Microliter
Preservative
Yes
Purification Method
IEX

Storage & Handling

Storage Conditions
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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