LSBio
Polyclonal Rabbit anti-Bovine CA2 / Carbonic Anhydrase II Antibody (Liquid, Biotin, WB)

ELISA results of purified Rabbit Anti-CARBONIC ANHYDRASE II Biotin Conjugated Antibody tested against Carbonic Anhydrase II. Each well was coated in duplicate with 1.0 µg of Carbonic Anhydrase II. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer MB-060-1000, Streptavidin HRP conjugate, and TMB-1000 substrate.

Polyclonal Rabbit anti-Bovine CA2 / Carbonic Anhydrase II Antibody (Liquid, Biotin, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity:Bovine
Format:Liquid
See all Bovine CA2 / Carbonic Anhydrase II antibodies →
$311.00each
Catalog Number: LS-C745133-25

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Overview

Description
Carbonic Anhydrase II antibody LS-C745133 is a biotin-conjugated rabbit polyclonal antibody to bovine Carbonic Anhydrase II (CA2). Validated for ELISA and WB.
Application
ELISA, WB
Reactivity
Bov
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
CA-II | CAII | CAC | Car2 | Carbonate dehydratase II | Carbonic anhydrase C | Carbonic anhydrase II | Carbonic dehydratase | CA2 | Carbonic anhydrase 2 | Carbonic anhydrase B
Recommended Dilution: ELISA
1:2000 - 1:10000
Recommended Dilution: WB
1:200 - 1:1000
UniProt Number
P00918
SwissProt
P00918

Target

Target
Bovine CA2 / Carbonic Anhydrase II
Antigen Species
Bov
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Carbonic Anhydrase II [Bovine Erythrocytes]. Cross reactivity against Carbonic Anhydrase II from other sources may occur but has not been specifically determined.
Gene Name
CA2

Antibody

Host Species
Rb
Clonality
Polyclonal
Isotype
IgG
Origin Species
Hu

Immunogen

Immunogen
Carbonic Anhydrase II [Bovine Erythrocytes].

Format

Conjugate/Label
Biotin
Format
Liquid
Formulation
10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Volume
25 Microliter
Preservative
Yes
Purification Method
IEX

Storage & Handling

Storage Conditions
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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