Products
Research Areas
COVID-19
Resources
Contact Us
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Cart
How To Buy
Contact Us

Locations

Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464

Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers


Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

Contact Us


Additional Contact Details

Distributors
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Antibodies > BUB1 > LS-C343995
 
Print Page  
Catalog Number Size Price
LS-C343995-10 10 µg $318 
LS-C343995-100 100 µg (0.5 mg/ml) $470 
BUB1 Antibody - Western blot analysis of BUB1 using anti-BUB1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: human Caco-2 whole cell lysates, Lane 6: human PC-3 whole cell lysates, Lane 7: human MCF-7 whole cell lysates, Lane 8: human U2OS whole cell lysates, Lane 9: rat testis tissue lysates, Lane 10: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BUB1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for BUB1 at approximately 130KD. The expected band size for BUB1 is at 122KD.
BUB1 Antibody - Bub1 antibody Western blot. All lanes: Anti BUB1 at 0.5 ug/ml. WB: Recombinant Human BUB1 Protein 0.5ng. Predicted band size: 39 kD. Observed band size: 39 kD.
BUB1 Antibody - Western blot analysis of BUB1 using anti-BUB1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: human Caco-2 whole cell lysates, Lane 6: human PC-3 whole cell lysates, Lane 7: human MCF-7 whole cell lysates, Lane 8: human U2OS whole cell lysates, Lane 9: rat testis tissue lysates, Lane 10: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BUB1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for BUB1 at approximately 130KD. The expected band size for BUB1 is at 122KD.
BUB1 Antibody - Bub1 antibody Western blot. All lanes: Anti BUB1 at 0.5 ug/ml. WB: Recombinant Human BUB1 Protein 0.5ng. Predicted band size: 39 kD. Observed band size: 39 kD.
1 of 2
2 of 2

Polyclonal Rabbit anti‑Human BUB1 Antibody (aa731‑1085, WB) LS‑C343995

Polyclonal Rabbit anti‑Human BUB1 Antibody (aa731‑1085, WB) LS‑C343995

Submit Review LSBio Guarantee
Note: This antibody replaces LS-C388073
Antibody:
BUB1 Rabbit anti-Human Polyclonal (aa731-1085) Antibody
Application:
WB
Reactivity:
Human, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C343995-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
BUB1 Rabbit anti-Human Polyclonal (aa731-1085) Antibody
Application:
WB
Reactivity:
Human, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
BUB1 antibody LS-C343995 is an unconjugated rabbit polyclonal antibody to BUB1 (aa731-1085) from human. It is reactive with human and rat. Validated for WB.
Target
Human BUB1
Synonyms
BUB1 | Bub1A | BUB1L | HBUB1
Host
Rabbit
Reactivity
Human, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived human Bub1 recombinant protein (Position: V731-K1085). Human Bub1 shares 81% amino acid (aa) sequence identity with mouse Bub1.
Epitope
aa731-1085
Specificity
Expressed in metastatic melanoma, liver, skin, kidney, heart, lung, lymph node, skeletal muscle and brain, and also in A2058 melanoma cells and activated T-cells (at protein level). Expressed in blood vessels. Strongly expressed in endothelial cells, cytotrophoblasts, and poorly differentiated. colon adenocarcinoma cells found in lymphatics. .
Applications
  • Western blot
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BUB1
O43683 NM_004336 NP_004327.1

Publications (0)

Submit a Publication for LS-C343995

Customer Reviews (0)

Submit a review for LS-C343995

Featured Products

BUB1 Antibody - Anti-BUB1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC‑plus™ BUB1 Antibody LS‑B2108
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA
BUB1 Antibody - Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using BUB1 Antibody. The picture on the right is blocked with the synthesized peptide.
BUB1 Antibody (aa251‑300) LS‑C118685
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Peptide Enzyme-Linked Immunosorbent Assay
BUB1 Antibody - BUB1A Antibody western blot of A549 cell line lysates (35 ug/lane). The BUB1A antibody detected the BUB1A protein (arrow).
BUB1 Antibody (aa1‑30) LS‑C156894
Species: Human
Applications: Western blot, Flow Cytometry
BUB1 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human BUB1 Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G80179
Source: Human
Tag: Myc-DDK (Flag)
AHR Antibody - IHC staining of FFPE rat small intestine with AHR antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
AHR Antibody LS‑C783005
Species: Human, Mouse, Rat
Applications: IHC - Paraffin, Western blot, Flow Cytometry
CRLF1 ELISA Kit
Mouse CRLF1 (Sandwich ELISA) ELISA Kit - LS-F9369
Reactivity: Mouse
Range: 15.6-1000 pg/ml
See all BUB1 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/23/2024
Family of Companies
Site Links Home All Antibodies How To Buy All Proteins Distributors All ELISA & Assay Kits Reviews PathPlus™ Antibodies
Follow LSBio View Cart Register or Login
Copyright © 2022 LifeSpan BioSciences, Inc - Privacy Policy
Contact Us

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464

Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

To place an order:
Orders@LSBio.com

For questions about orders:
Customer.Support@LSBio.com

For technical assistance:
Technical.Support@LSBio.com