LSBio
Biotin Antibody (clone 4C7.D5)

Immunofluorescence Microscopy of Mouse Anti-Biotin antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: Biotinylated anti-transferrin receptor antibody. Secondary antibody: CF®488A anti-biotin secondary antibody at 2ug/mL for 45 min at RT. Localization/Staining: plasma membrane and endosome.

Biotin Antibody (clone 4C7.D5)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Western Blot
Format:Liquid
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$408.00each
Catalog Number: LS-C153773-100

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Overview

Description
Biotin antibody LS-C153773 is an unconjugated mouse monoclonal antibody to Biotin. Validated for ELISA, IF and WB.
Application
ELISA, IF, WB
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Recommended Dilution: ELISA
1:20000
Recommended Dilution: WB
1:2000 - 1:10000

Target

Target
Biotin
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-mouse serum, Biotin conjugated IgG and Biotin conjugated Bovine Serum Albumin.

Antibody

Host Species
Ms
Clonality
Monoclonal
Clone
4C7.D5
Isotype
IgG2a
Light Chain
λ

Immunogen

Immunogen
Anti-Biotin Antibody was produced by repeated immunizations with Biotin conjugated to Keyhole Limpet Hemocyanin (b-KLH).

Format

Conjugate/Label
Unconj
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Prot A

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.