LSBio
Polyclonal Rabbit anti-Human BAD Antibody (phospho-Ser99, IHC, IF, WB)

Fluorescent confocal image of HeLa cells stained with phospho-Bad-S99 antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated phospho-Bad-S99 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the phospho-Bad-S99 mainly to the cytoplasm.

Polyclonal Rabbit anti-Human BAD Antibody (phospho-Ser99, IHC, IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Dot Blot, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human
Format:Liquid
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$401.00each
Catalog Number: LS-C97419-400

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Overview

Description
BAD antibody LS-C97419 is an unconjugated rabbit polyclonal antibody to human BAD (pSer99). Validated for DB, IF, IHC and WB.
Application
DB, IF, IHC, IHC-P, WB
Reactivity
Hu
Predicted Reactivity
Ms, Rt
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
BBC2 | Bcl-2-like protein 8 | BCL2-binding component 6 | BCL2L8 | BBC6 | BCL-X/BCL-2 binding protein | Bcl-2-binding component 6 | BAD | Bcl2 antagonist of cell death | BCL2-binding protein | Bcl2-L-8
Recommended Dilution: IF/ICC
1:200
Recommended Dilution: IHC-P
1:50 - 1:100
Recommended Dilution: WB
1:1000
UniProt Number
Q92934
SwissProt
Q92934

Target

Target
Human BAD
Antigen Species
Hu
Epitope
This Bad Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S99 of human Bad.
Gene Name
BAD

Antibody

Host Species
Rb
Clonality
Polyclonal
Origin Species
Hu

Immunogen

Immunogen Type
SP-Lin

Format

Conjugate/Label
Unconj
Format
Liquid
Formulation
PBS, 0.09% Sodium Azide
Volume
400 Microliter
Preservative
Yes
Purification Method
Prot A

Storage & Handling

Storage Conditions
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Recommended Storage Buffer
PBS

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