LSBio
Polyclonal Rabbit anti-Human B2M / Beta 2 Microglobulin Antibody (Liquid, Biotin, IHC, WB)

Dot Blot results of rabbit Anti-Beta 2 Microglobulin Biotin Conjugated. Antigen: Beta-2-Microglobulin. Blot loaded at 3 fold dilution: 1. 100ng, 2. 33.3ng, 3. 11.1ng, 4. 3.70ng, 5. 1.23ng. Blocking: MB-070 Buffer for 30 minutes at RT. Primary Antibody: Rabbit Anti-Beta-2-Microglobulin Biotin 10µg/mL for 1hr at RT. Secondary Antibody: Streptavidin-HRP at 1:40,000 for 30min at RT. Imaging System ChemiDoc, Filter used: Chemi.

Polyclonal Rabbit anti-Human B2M / Beta 2 Microglobulin Antibody (Liquid, Biotin, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Dot Blot, Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:Human
Format:Liquid
See all Human B2M / Beta 2 Microglobulin antibodies →
$311.00each
Catalog Number: LS-C745052-25

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Overview

Description
Beta 2 Microglobulin antibody LS-C745052 is a biotin-conjugated rabbit polyclonal antibody to human Beta 2 Microglobulin (B2M). Validated for DB, ELISA, IHC and WB.
Application
DB, ELISA, IHC, WB
Reactivity
Hu
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
B2M | Beta 2 Microglobulin | Beta-2-microglobulin | Beta-2-microglobin
Recommended Dilution: ELISA
1:2000 - 1:10000
Recommended Dilution: IHC
1:500 - 1:3000
Recommended Dilution: WB
1:1000 - 1:5000
UniProt Number
P61769
SwissProt
P61769

Target

Target
Human B2M / Beta 2 Microglobulin
Antigen Species
Hu
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified b2-Microglobulin [Human Urine]. Cross reactivity against b2-Microglobulin from other sources may occur but has not been specifically determined.
Gene Name
B2M

Antibody

Host Species
Rb
Clonality
Polyclonal
Isotype
IgG
Origin Species
Hu

Immunogen

Immunogen
Anti-Beta-2-Microglobulin Antibody was produced by repeated immunizations with beta-2-Microglobulin protein isolated from human urine.
Immunogen Type
Native

Format

Conjugate/Label
Biotin
Format
Liquid
Formulation
10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Volume
25 Microliter
Preservative
Yes
Purification Method
IEX

Storage & Handling

Storage Conditions
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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