Polyclonal Rabbit anti-Human SLC5A11 / SMIT2 Antibody (WB)
Catalog Number: LS-C748223
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Fluorescent image of U251 cells stained with ATG12 antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated ATG12 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG12 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
Polyclonal Rabbit anti-Human APG12 / ATG12 Antibody (aa1-30, IHC, IF, WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$401.00each
Catalog Number: LS-C156569-400
Catalog Number: LS-C156569
For bulk orders or custom formulations:
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- Description
- ATG12 antibody LS-C156569 is an unconjugated rabbit polyclonal antibody to human ATG12 (APG12) (aa1-30). Validated for IF, IHC and WB. Cited in 1 publication.
- Application
- IF, IHC, IHC-P, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Autophagy-related protein 12 | APG12L | Autophagy related 12 | ATG12 | FBR93 | APG12 | APG12-like | Ubiquitin-like protein ATG12 | HAPG12
- Recommended Dilution: IF/ICC
- 1:200
- Recommended Dilution: IHC-P
- 1:50 - 1:100
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- O94817
- NCBI GenBank
- NM_004707 | NP_004698.3
- SwissProt
- O94817
Target
- Target
- Human APG12 / ATG12
- Antigen Species
- Human
- Epitope
- This ATG12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human ATG12.
- Gene Name
- ATG12
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen Type
- Peptide
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.09% Sodium Azide
- Volume
- 400 Microliter
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
- Recommended Storage Buffer
- PBS
Activation of autophagy in mesenchymal stem cells provides tumor stromal support. Sanchez CG, Penfornis P, Oskowitz AZ, Boonjindasup AG, Cai DZ, Dhule SS, Rowan BG, Kelekar A, Krause DS, Pochampally RR. Carcinogenesis. 2011;32:964-72. PubMed: 21317300 PMC: PMC3128555
