LSBio
Polyclonal Rabbit anti-Human APAF1 / APAF-1 Antibody (aa12-28, IHC, WB)

Western blot of whole cell lysate from K562 cells probed with Rabbit anti-APAF1 (RABBIT ANTI APAF1 (aa12-28)) either in the absence (A) or presence (B) of blocking peptide.

Polyclonal Rabbit anti-Human APAF1 / APAF-1 Antibody (aa12-28, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human
Format:Liquid
See all Human APAF1 / APAF-1 antibodies →
$463.00each
Catalog Number: LS-C42802-0.1

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Overview

Description
APAF-1 antibody LS-C42802 is an unconjugated rabbit polyclonal antibody to human APAF-1 (APAF1) (aa12-28). Validated for IHC and WB.
Application
IHC, IHC-P, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
APAF-1 | APAF1 | CED4 | KIAA0413
Recommended Dilution: WB
1:1000 - 1:2000
UniProt Number
O14727
NCBI GenBank
NM_013229 | NP_037361.1
SwissProt
O14727

Target

Target
Human APAF1 / APAF-1
Antigen Species
Human
Epitope
Apaf1 (Apoptosis protease activating factor 1) is the mammalian homologue of the CED-4 cell death protein in C. Elegans. Apaf1 binds to cytochrome C and caspase-9 leading to caspase-9 activation, resulting in the beginning of the proteolytic cascade characteristic of apoptosis. Apaf1 can also associate with caspase-4 and caspase-8. AHP487 recognizes a 130kD band in Western blots.
Gene Name
APAF1

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Synthetic peptide corresponding to amino acids 12-28 of human Apaf1 (AA 12-28).
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, 0.02% Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Recommended Storage Buffer
PBS

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