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DCAF7 / WDR68 Antibody (N‑Terminus) LS‑C135268
WDR68 antibody LS-C135268 is an unconjugated rabbit polyclonal antibody to WDR68 (DCAF7) from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C135268-50
50 µg
Unavailable

Popular DCAF7 / WDR68 Products

Antibody
Species: Human
Applications: Western blot, ELISA
Antibody
Species: Human
Applications: Western blot, ELISA
Immunofluorescence analysis of U2OS cells.
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot
Immunohistochemistry of paraffin-embedded human colon cancer using antibody at 1:100 dilution.
Species: Human
Applications: IHC, IHC - Paraffin, ELISA
Immunofluorescent analysis of DCAF7 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Species: Human, Mouse, Rat
Applications: ICC, Immunofluorescence, Western blot

Product Description

WDR68 antibody LS-C135268 is an unconjugated rabbit polyclonal antibody to WDR68 (DCAF7) from human, mouse, rat and other species. Validated for WB.
About DCAF7 / WDR68
Involved in craniofacial development. Acts upstream of the EDN1 pathway and is required for formation of the upper jaw equivalent, the palatoquadrate. The activity required for EDN1 pathway function differs between the first and second arches (By similarity). Associates with DIAPH1 and controls GLI1 transcriptional activity. Could be involved in normal and disease skin development. May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex. P61962 NM_005828 NP_005819.3

DCAF7 Antibody, Human anthocyanin Antibody, AN11 Antibody, WD-repeat protein Antibody, WD repeat domain 68 Antibody, HAN11 Antibody, SWAN-1 Antibody, WDR68 Antibody


Specifications

Target
Mouse DCAF7 / WDR68
Host
Rabbit
Reactivity
Mouse, Human, Monkey, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish, Drosophila (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
DCAF7 / WDR68 antibody was raised against synthetic peptide from N-Terminus of mouse Dcaf7 (P61963, NP_082222). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Zebra finch, Chicken, Lizard, Xenopus, Salmon, Smelt, Zebrafish, Sea squirt, Drosophila (100%); Beetle (90%).
Blocking Peptide
LS-E18710 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C135268. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
N-Terminus
Specificity
Mouse DCAF7 / WDR68
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

DCAF7 / WDR68 antibody LS-C135268 Western blot of Mouse Spleen lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
DCAF7 / WDR68 antibody LS-C135268 Western blot of Mouse Spleen lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

DCAF7 / WDR68 antibody LS-C135268 Western blot of Mouse Spleen lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
DCAF7 / WDR68 antibody LS-C135268 Western blot of Mouse Spleen lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

DCAF7 / WDR68 antibody LS-C135268 Western blot of Mouse Spleen lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
DCAF7 / WDR68 antibody LS-C135268 Western blot of Mouse Spleen lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

DCAF7 / WDR68 antibody LS-C135268 Western blot of Mouse Spleen lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
DCAF7 / WDR68 antibody LS-C135268 Western blot of Mouse Spleen lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/17/2018

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