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Anti-DARS Antibody (clone 2F11) IHC-plus™ LS-B4310

Note: This antibody replaces LS-C104931

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular DARS Antibodies

Anti-DARS Antibody (clone 2F11) IHC-plus™ LS-B4310
Mouse Monoclonal [clone 2F11] (IgG2a,k) to Human DARS
Human
IHC - Paraffin, Western blot, ELISA, RNA interference
Unconjugated
Immunohistochemistry Image
Anti-DARS Antibody (aa154-183) LS-C167270
Rabbit Polyclonal to Human DARS
Human
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-DARS Antibody (aa396-445) IHC-plus™ LS-B12047
Rabbit Polyclonal to Human DARS
Human, Monkey, Mouse, Rat, Bovine, Guinea pig, Horse, Pig, Rabbit, Sheep
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 2F11] (IgG2a,k) to Human DARS
Human
IHC - Paraffin, Western blot, ELISA, RNA interference
Unconjugated

Details

Human DARS
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal [2F11]
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot
  • ELISA
  • RNA interference

Specificity and Use

DARS antibody was raised against dARS (NP_001340, 393 a.a. ~ 501 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Western Blot (Cell lysate) - positive control HeLa. Western Blot (Transfected lysate) - positive control transfected 293T cell line. Sandwich ELISA (Recombinant protein). RNAi Knockdown (Antibody validated).

Packaging

PBS, pH 7.4. Sourced in Ascites.
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
For research use only.

About DARS

P14868 NM_001349 NP_001340.2

DARS Antibody, Aspartyl-tRNA synthetase Antibody, AspRS Antibody

Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.

Immunohistochemistry

Immunohistochemistry
Anti-DARS antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4310 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-DARS antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4310 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-DARS antibody IHC of human ganglion cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4310 concentration 5 ug/ml.

Western blot

Western blot
DARS monoclonal antibody, clone 2F11 Western blot of DARS expression in HeLa.

Western blot

Western blot
Western blot of DARS expression in transfected 293T cell line by DARS monoclonal antibody, clone 2F11.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged DARS is approximately 0.3 ng/ml as a capture antibody.

RNA interference

RNA interference
Western blot of DARS over-expressed 293 cell line, cotransfected with DARS Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with DARS monoclonal antibody, clone 2F11. GAPDH ( 36.1 kD ) used as specificity and.
 

Requested From: United States
Date Requested: 12/3/2016

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