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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CYCS / Cytochrome c Antibody (clone 6H2.B4) LS‑C513
Cytochrome c antibody LS-C513 is an unconjugated mouse monoclonal antibody to Cytochrome c (CYCS) from human, mouse and rat. Validated for IHC, IP and Neut.
Catalog
Size
Price
LS-C513-100
100 µg (0.5 mg/ml)
Unavailable

Popular CYCS / Cytochrome c Products

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Applications: IHC, IHC - Paraffin, Western blot

Product Description

Cytochrome c antibody LS-C513 is an unconjugated mouse monoclonal antibody to Cytochrome c (CYCS) from human, mouse and rat. Validated for IHC, IP and Neut.
About CYCS / Cytochrome c
Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.Plays a role in apoptosis. P99999 NM_018947 NP_061820.1

CYCS Antibody, CYC Antibody, HCS Antibody, THC4 Antibody, Cytochrome c Antibody, Cytochrome c, somatic Antibody


Specifications

Target
Rat CYCS / Cytochrome c
Host
Mouse
Reactivity
Rat, Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [6H2.B4]
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Frozen (2 - 4 µg/ml)
  • Immunoprecipitation
  • Neutralization
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Failed Applications
  • IHC - Paraffin
Immunogen
Recombinant rat CYCS / Cytochrome c.
Specificity
Rat cytochrome c was used as immunogen to generate 6H2. B4 hybridoma clone (Goshorn et al, 1991). The antibody recognizes an epitope around residue 62 of rat Cytochrome c.
Presentation
PBS, 0.2%gelatin, 0.05% sodium azide.
Storage
4°C or -20°C, Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Immunoprecipitation

Detection of cytochrome c in HeLa cells: Two micrograms of 6H2. B4 (lane 1) or control IgG (lane 2) was used to immunoprecipitate cytochrome c from 1 x 10^6 cells and the immunoprecipitated protein was resolved on SDS-PAGE. Cytochrome c was detected by WB.
Detection of cytochrome c in HeLa cells: Two micrograms of 6H2. B4 (lane 1) or control IgG (lane 2) was used to immunoprecipitate cytochrome c from 1 x 10^6 cells and the immunoprecipitated protein was resolved on SDS-PAGE. Cytochrome c was detected by WB.

Immunoprecipitation

Detection of cytochrome c in HeLa cells: Two micrograms of 6H2. B4 (lane 1) or control IgG (lane 2) was used to immunoprecipitate cytochrome c from 1 x 10^6 cells and the immunoprecipitated protein was resolved on SDS-PAGE. Cytochrome c was detected by WB.
Detection of cytochrome c in HeLa cells: Two micrograms of 6H2. B4 (lane 1) or control IgG (lane 2) was used to immunoprecipitate cytochrome c from 1 x 10^6 cells and the immunoprecipitated protein was resolved on SDS-PAGE. Cytochrome c was detected by WB.

Immunoprecipitation

Detection of cytochrome c in HeLa cells: Two micrograms of 6H2. B4 (lane 1) or control IgG (lane 2) was used to immunoprecipitate cytochrome c from 1 x 10^6 cells and the immunoprecipitated protein was resolved on SDS-PAGE. Cytochrome c was detected by WB.
Detection of cytochrome c in HeLa cells: Two micrograms of 6H2. B4 (lane 1) or control IgG (lane 2) was used to immunoprecipitate cytochrome c from 1 x 10^6 cells and the immunoprecipitated protein was resolved on SDS-PAGE. Cytochrome c was detected by WB.

Immunoprecipitation

Detection of cytochrome c in HeLa cells: Two micrograms of 6H2. B4 (lane 1) or control IgG (lane 2) was used to immunoprecipitate cytochrome c from 1 x 10^6 cells and the immunoprecipitated protein was resolved on SDS-PAGE. Cytochrome c was detected by WB.
Detection of cytochrome c in HeLa cells: Two micrograms of 6H2. B4 (lane 1) or control IgG (lane 2) was used to immunoprecipitate cytochrome c from 1 x 10^6 cells and the immunoprecipitated protein was resolved on SDS-PAGE. Cytochrome c was detected by WB.

Requested From: 
Date Requested: 7/15/2018

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