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Catalog Number Size Price
LS-B362-50 50 µl (85 mg/ml) $515 
CUL7 Antibody - Anti-CUL7 / Cullin 7 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.

IHC‑plus™ Polyclonal Rabbit anti‑Human CUL7 Antibody (aa1675‑1698, IHC, WB) LS‑B362

IHC‑plus™ Polyclonal Rabbit anti‑Human CUL7 Antibody (aa1675‑1698, IHC, WB) LS‑B362

Note: This antibody replaces LS-C18820
Antibody:
CUL7 Rabbit anti-Human Polyclonal (aa1675-1698) Antibody
Application:
IHC, IHC-P, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$515
LS-B362-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CUL7 Rabbit anti-Human Polyclonal (aa1675-1698) Antibody
Application:
IHC, IHC-P, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
CUL7 antibody LS-B362 is an unconjugated rabbit polyclonal antibody to human CUL7 (aa1675-1698). Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
Target
Human CUL7
Synonyms
CUL7 | Cullin 7 | Cullin-7 | CUL-7 | DJ20C7.5 | KIAA0076
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Delipidated and defibrinated followed by sterile filtration
Modifications
Unmodified
Immunogen
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminus region near amino acids 1675-1698 of Human Cul7 coupled to KLH.
Epitope
aa1675-1698
Specificity
Reacts with human and mouse Cullin 7. Cross reactivity with other human cullins may occur.
Applications
  • IHC
  • IHC - Paraffin (1:500)
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation
  • ELISA (1:2000 - 1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B362 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B362 was determined to be 1:500.
Presentation
Antiserum, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CUL7
Q14999 NM_014780 NP_055595.2

Validation

CUL7 Antibody - Anti-CUL7 / Cullin 7 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
Anti-CUL7 / Cullin 7 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
Anti-CUL7 / Cullin 7 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.

LSBio Ratings

IHC-plus™ CUL7 Antibody (aa1675-1698) for IHC, WB/Western, IP, ELISA LS-B362 has an LSBio Rating of
Publications (4.1)
Laboratory Validation Score (4)

Publications (2)

CUL7: A DOC domain-containing cullin selectively binds Skp1.Fbx29 to form an SCF-like complex. Dias DC, Dolios G, Wang R, Pan ZQ. Proceedings of the National Academy of Sciences of the United States of America. 2002 99:16601-6.

Targeted disruption of p185/Cul7 gene results in abnormal vascular morphogenesis. Arai T, Kasper JS, Skaar JR, Ali SH, Takahashi C, DeCaprio JA. Proceedings of the National Academy of Sciences of the United States of America. 2003 100:9855-60.

Customer Reviews (0)


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Requested From: United States
Date Requested: 4/18/2024