Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
Western blot (1:200 - 1:2000)
ELISA (1:2000 - 1:10000)
IHC - Paraffin
Specificity and Use
CTCFL / BORIS antibody was raised against synthetic peptide from human CTCFL / BORIS.
aa 9-26 of human BORIS protein.
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band approximately 75 kD in size corresponding to BORIS by western blotting in the appropriate cell lysate or extract.
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
CTCFL Antibody, BORIS Antibody, CCCTC-binding factor Antibody, Cancer/testis antigen 27 Antibody, BORIS-like protein Antibody, CT27 Antibody, CTCF-like protein Antibody, CTCF-T Antibody, CTCF paralog Antibody, DJ579F20.2 Antibody, HMGB1L1 Antibody, Zinc finger protein CTCF-T Antibody, HMG-1L1 Antibody
Testis-specific DNA binding protein responsible for insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. Plays a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2.
Anti-BORIS Antibody - Western Blot. Western blot of Affinity Purified anti-BORIS antibody shows detection of a predominant band corresponding to BORIS in human tissue lysates (arrowhead). Lane 1 contains lysate from human prostate tissue. Lane 2 contains lysate from human spleen tissue. A predominant band at ~75 kD is observed. Molecular weight estimation was made by comparison to prestained MW markers as indicated.
Requested From: United States
Date Requested: 2/22/2017