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Anti-COPB2 / Beta-COP Antibody (aa494-511) IHC-plus™ LS-B2677

Note: This antibody replaces LS-C14587, LS-C14589

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular COPB2 / Beta-COP Antibodies

Anti-COPB2 / Beta-COP Antibody (aa494-511) IHC-plus™ LS-B2677
Rabbit Polyclonal (IgG) to Rat COPB2 / Beta-COP
Rat, Human, Monkey, Mouse, Bat, Bovine, Dog, Horse, Pig
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-COPB2 / Beta-COP Antibody (aa814-843) LS-C159392
Rabbit Polyclonal to Human COPB2 / Beta-COP
Human, Mouse
Western blot
Unconjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Rat COPB2 / Beta-COP
Rat, Human, Monkey, Mouse, Bat, Bovine, Dog, Horse, Pig
IHC - Paraffin, Western blot
Unconjugated

Details

Rat COPB2 / Beta-COP
Rabbit
Rat, Human, Monkey, Mouse, Bat, Bovine, Dog, Horse, Pig (tested or 100% immunogen sequence identity)
Rabbit (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (2 µg/ml)

Specificity and Use

COPB2 / Beta-COP antibody was raised against synthetic peptide: KVLAAQETHEGVTEDGIE, corresponding to amino acids 494-511 of Rat beta COP I. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Bovine, Horse, Pig, Opossum, Platypus (100%); Orangutan, Rabbit, Lizard (94%); Chicken (89%); Turkey, Xenopus, Sea anemone (83%).
aa494-511
Detects rat coatomer-protein I beta (COP I beta).
Immunohistochemistry: LS-B2677 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2677 was determined to be 5 ug/ml. Western Blot: 2 ug/ml; Detects an ~115kD protein representing COP I beta from PC-12 cell extract.

Packaging

PBS, 1 mg/ml BSA, 0.05% sodium azide.
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
For research use only.

About COPB2 / Beta-COP

P35606 NM_004766 NP_004757.1

COPB2 Antibody, Betaprime-COP Antibody, Beta'-COP Antibody, Beta-coat protein Antibody, Beta-COP Antibody, p102 Antibody, Coatomer beta' subunit Antibody, Coatomer subunit beta Antibody

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.

Immunohistochemistry

Immunohistochemistry
Anti-COPB2 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2677 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-COPB2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2677 concentration 5 ug/ml.

Requested From: United States
Date Requested: 12/6/2016

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