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Anti-Collagen VI Antibody (Biotin) IHC-plus™ LS-B380

Note: This antibody replaces LS-C18776

Ordering

Wt. Vol. Conc. Price
100 µg 100 µl 1 mg/ml $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular Collagen VI Antibodies

Anti-Collagen VI Antibody (Biotin) IHC-plus™ LS-B380
Rabbit Polyclonal to Human Collagen VI
Human, Bovine
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Biotin Conjugated
Immunohistochemistry Image
Anti-Collagen VI Antibody IHC-plus™ LS-B696
Rabbit Polyclonal to Human Collagen VI
Human, Bovine
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image
Anti-Collagen VI Antibody LS-C79649
Mouse Monoclonal (IgG1,k)
Human, Rabbit
IHC - Frozen, Western blot, ELISA
Unconjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human Collagen VI
Human, Bovine
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Biotin Conjugated

Details

Human Collagen VI
Rabbit
Human, Bovine (tested or 100% immunogen sequence identity)
Polyclonal
Biotin
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (1.25 µg/ml)
  • Western blot (1:5000 - 1:10000)
  • Immunoprecipitation
  • ELISA (1:10000 - 1:50000)

Specificity and Use

Collagen VI antibody was raised against collagen Type VI from human and bovine placenta
Collagen Type VI from human and bovine placenta.
Recent testing with this antibody has shown that it has some cross reactivity with COL6A2. Immunohistochemistry: LS-B380 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B380 was determined to be 1.25 ug/ml. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

0.125 M sodium borate, 0.075 M sodium chloride, 0.005 M EDTA;, pH 8.0, 0.01% sodium azide, 10 mg/ml BSA (Immunoglobulin, protease Free).
100 µl Sterile ddH2O
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze/thaw cycles. Store undiluted.
For research use only.

About Collagen VI

Collagen VI is a form of collagen primarily associated with the extracellular matrix of skeletal muscle. It is associated with the genes COL6A1, COL6A2, COL6A3 and COL6A5. Defects are associated with Bethlem myopathy and Ullrich congenital muscular dystrophy.

Immunohistochemistry

Immunohistochemistry
Anti-Collagen VI antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B380 concentration 1.25 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
Anti-Collagen VI antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B380 concentration 1.25 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 2/28/2017

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