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Anti-CEB1 / HERC5 Antibody (aa900-949) IHC-plus™ LS-B9697

Note: This antibody replaces LS-C102007

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl 1 mg/ml $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CEB1 / HERC5 Antibodies

Anti-CEB1 / HERC5 Antibody (aa900-949) IHC-plus™ LS-B9697
Rabbit Polyclonal (IgG) to Human CEB1 / HERC5
Human, Monkey, Bovine, Horse, Rabbit
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-CEB1 / HERC5 Antibody (aa287-336) IHC-plus™ LS-B12707
Rabbit Polyclonal to Human CEB1 / HERC5
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry - Paraffin Image
Anti-CEB1 / HERC5 Antibody LS-C411070
Rabbit Polyclonal (IgG) to Human CEB1 / HERC5
Human
Western blot
Unconjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human CEB1 / HERC5
Human, Monkey, Bovine, Horse, Rabbit
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human CEB1 / HERC5
Rabbit
Human, Monkey, Bovine, Horse, Rabbit (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

CEB1 / HERC5 antibody was raised against synthetic peptide located between aa900-949 of human HERC5 (Q69G20, NP_057407). Percent identity by BLAST analysis: Human, Gibbon, Monkey (100%); Gorilla (92%); Rabbit (85%).
aa900-949
Human HERC5
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About CEB1 / HERC5

Q9UII4 NM_016323 NP_057407.2

HERC5 Antibody, CEB1 Antibody, CEBP1 Antibody, Cyclin-E-binding protein 1 Antibody, E3 ISG15--protein ligase HERC5 Antibody, Hect domain and RLD 5 Antibody

Major E3 ligase for ISG15 conjugation. Acts as a positive regulator of innate antiviral response in cells induced by interferon. Functions as part of the ISGylation machinery that recognizes target proteins in a broad and relatively non-specific manner. Catalyzes ISGylation of IRF3 which results in sustained activation, it attenuates IRF3-PIN1 interaction, which antagonizes IRF3 ubiquitination and degradation, and boosts the antiviral response.

Immunohistochemistry

Immunohistochemistry
Anti-CEB1 / HERC5 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B9697 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
HERC5 antibody Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/5/2016

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