Mouse CCL20 / MIP-3-Alpha
(tested or 100% immunogen sequence identity)
Purified, heated to 56°C for 30 minutes
- Western blot (1:2000 - 1:10000)
MIP-3 alpha Antibody Peroxidase Conjugated was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant mouse MIP-3a protein.
Mouse MIP-3 alpha Antibody Peroxidase Conjugated was heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant mouse MIP-3a in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
This purified antibody has been tested for use in ELISA and western blotting. By western blot a band approximately 10.7 kD in size corresponding to mouse MIP-3a protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 1% BSA, 0.01% Gentamicin sulfate
Reconstitute with 100ul sterile ddH2O
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze/thaw cycles. Store undiluted.
For research use only.
This antibody carries the LSBio 100% Guarantee.