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Anti-CAMK4 / CaMK IV Antibody (aa305-323) IHC-plus™ LS-B335

Note: This antibody replaces LS-C18758

Ordering

Wt. Vol. Conc. Price
- 50 µl 70 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CAMK4 / CaMK IV Antibodies

Anti-CAMK4 / CaMK IV Antibody (aa305-323) IHC-plus™ LS-B335
Rabbit Polyclonal to Human CAMK4 / CaMK IV
Human, Mouse, Rat, Bovine, Dog
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-CAMK4 / CaMK IV Antibody (clone 1A3) IHC-plus™ LS-B6013
Mouse Monoclonal [clone 1A3] (IgG1,k) to Human CAMK4 / CaMK IV
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-CAMK4 / CaMK IV Antibody (phospho-Thr196/Thr200) LS-C198721
Rabbit Polyclonal (IgG) to Human CAMK4 / CaMK IV
Human, Mouse, Rat
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human CAMK4 / CaMK IV
Human, Mouse, Rat, Bovine, Dog
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human CAMK4 / CaMK IV
Rabbit
Human, Mouse, Rat, Bovine, Dog (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Delipidated and defibrinated
Unmodified

Applications

  • IHC - Paraffin (1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:2000)
  • ELISA (1:5000 - 1:25000)

Specificity and Use

CAMK4 / CaMK IV antibody was raised against synthetic peptide from human CAMK4.
aa305-323
Amino acids 305-323 of Human CaM Kinase IV protein.
Immunohistochemistry: LS-B335 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B335 was determined to be 1:500.

Packaging

PBS, pH 7.2, 0.01% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About CAMK4 / CaMK IV

Q16566 NM_001744 NP_001735.1

CAMK4 Antibody, CAM kinase IV Antibody, CaMK IV Antibody, CaMK-GR Antibody, CAMKIV Antibody, CaMK Antibody, CAM kinase- GR Antibody, CaM kinase-GR Antibody, CaM-KIV Antibody

Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK4 signaling cascade and regulates, mainly by phosphorylation, the activity of several transcription activators, such as CREB1, MEF2D, JUN and RORA, which play pivotal roles in immune response, inflammation, and memory consolidation. In the thymus, regulates the CD4+/CD8+ double positive thymocytes selection threshold during T-cell ontogeny.

Immunohistochemistry

Immunohistochemistry
Anti-CAMK4 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B335 dilution 1:500.

Western blot

Western blot
Anti-CaM Kinase IV Antibody - Western Blot. Western blot of Anti-CaM Kinase IV antibody shows detection of a band ~52 kD corresponding to CaM Kinase IV (arrowhead) in various preparations: lane 1 - rat brain lysate, lane 2 - Jurkat cell lysate. Specific reactivity is blocked in both lysates when antibody is preincubated with immunizing peptide (lanes 3 and 4 respectively). Approximately 35 ug of each lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. CaM Kinase IV was similarly detected on lysates from mouse brain (not shown). After blocking the membrane was probed with the primary antibody diluted to 1:1000 for 2h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.

Western blot

Western blot
Western blot of anti-CaM Kinase IV antibody shows detection of a band ~52 kDa corresponding to CaM Kinase IV (arrowhead) in various preparations. Staining of rat brain lysate is shown in lane 1. Jurkat cell lysate staining is shown in lane 2.

Western blot

Western blot
Specific reactivity is blocked in both lysates when antibody is preincubated with immunizing peptide (lanes 1 and 2 respectively).

Requested From: United States
Date Requested: 12/10/2016

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