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Anti-C/EBP Delta / CEBPD Antibody (N-Terminus) LS-C60129

Ordering

Wt. Vol. Conc. Price
100 µg - 1.33 mg/ml $405
Inquire for larger quantities

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Most Popular C/EBP Delta / CEBPD Antibodies

Anti-C/EBP Delta / CEBPD Antibody (N-Terminus) LS-C60129
Rabbit Polyclonal to Mouse C/EBP Delta / CEBPD
Mouse, Human
Western blot, ELISA
Unconjugated
Western blot Image
Anti-C/EBP Delta / CEBPD Antibody (aa169-197) LS-C168651
Rabbit Polyclonal to Human C/EBP Delta / CEBPD
Human, Mouse
Western blot
Unconjugated
Western blot Image
Anti-C/EBP Delta / CEBPD Antibody (C-Terminus) IHC-plus™ LS-B10190
Rabbit Polyclonal (IgG) to Human C/EBP Delta / CEBPD
Human, Mouse, Rat
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Mouse C/EBP Delta / CEBPD
Mouse, Human
Western blot, ELISA
Unconjugated

Details

Mouse C/EBP Delta / CEBPD
Rabbit
Mouse, Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • Western blot (1:1000 - 1:15000)
  • ELISA (1:4000 - 1:9000)

Specificity and Use

C/EBP Delta / CEBPD antibody was raised against recombinant protein corresponding to the amino terminus of mouse C/EBP.
N-Terminus
This antibody recognizes endogenous mouse and recombinant human C/EBP delta protein. A BLAST analysis was used to suggest cross-reactivity with C/EBP delta from human sources based on 77% homology with the immunizing sequence. No cross-reactivity is observed with mouse C/EBP alpha or C/EBP beta. Reactivity with C/EBP delta from other sources has not been determined
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 35 kD in size corresponding to C/EBPS by western blotting in the appropriate cell lysate or extract. The predicted molecular weight of mouse C/EBPS is 28.8 kD. This antibody is capable of detecting both over-expressed and endogenous C/EBPS.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Long term: -20°C; Short term: -20°C
For research use only.

About C/EBP Delta / CEBPD

P49716 NM_005195 NP_005186.2

CEBPD Antibody, C/EBP-delta Antibody, C/EBP delta Antibody, CELF Antibody, CRP3 Antibody, NF-IL6-beta Antibody, Nuclear factor NF-IL6-beta Antibody

C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers. Important transcriptional activator in the regulation of genes involved in immune and inflammatory responses, may play an important role in the regulation of the several genes associated with activation and/or differentiation of macrophages.

Western blot

Western blot
Anti-C/EBPd Antibody - Western Blot. Western blot of Anti-C/EBPd Antibody Lane 1: untreated wild-type (WT) cells Lane 2: WT cells treated 3 h with LPS Lane 3: C/EBPd-knock-out cells treated 3H with LPS Load: 35 ug per lane Primary: Anti-C/EBPd Antibody used at a dilution of 1:15k overnight at 4C Blocking: buffer TBST ECL was used for detection of endogenous protein in whole cell extracts from mouse primary macrophage cells. Predicted/Observed size: 28.8 kD, ~35 kD.

Western blot

Western blot
Anti-C/EBPd Antibody - Western Blot. Western blot of Anti-C/EBPd Antibody Lane 1: human C/EBPbeta Lane 2: Mouse C/EBPdelta Lane 3: untransfected Lane 4: human C/EBPdelta Load: 35 ug per lane Primary: Anti-C/EBPd Antibody used at a dilution of 1:15k overnight at 4C Blocking: buffer TBST ECL was used for detection of overexpressed protein in human and mouse C/EBP delta. Predicted/Observed size: 28.8 kD, ~35 kD.

Requested From: United States
Date Requested: 12/9/2016

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