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Anti-BUB1 Antibody (aa281-419) LS-C18935

Catalog Size Price
LS-C18935-100 100 µg Unavailable

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45 BUB1 Antibodies

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Anti-BUB1 Antibody (N-Terminus) IHC-plus™ LS-B2108
Rabbit Polyclonal (IgG) to Human BUB1
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IHC, IHC - Paraffin, Western blot
Anti-BUB1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2108 concentration 5 ug/ml.
Anti-BUB1 Antibody (aa251-300) LS-C118685
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IHC, IHC - Paraffin, ELISA
IHC of paraffin-embedded human breast carcinoma tissue, using BUB1 Antibody. The picture on the right is treated with the synthesized peptide.
Anti-BUB1 Antibody (aa1-30) LS-C156894
Rabbit Polyclonal to Human BUB1
Western blot, Flow Cytometry
BUB1A Antibody western blot of A549 cell line lysates (35 ug/lane). The BUB1A antibody detected the BUB1A protein (arrow).
Anti-BUB1 Antibody (clone 4C3-4C9) LS-C196778
Mouse Monoclonal [clone 4C3-4C9] (IgG1,k) to Human BUB1
Immunofluorescence, Western blot, ELISA
Immunofluorescence of monoclonal antibody to BUB1 on HeLa cell . [antibody concentration 10 ug/ml]
Anti-BUB1 Antibody (aa1-50) LS-C287268
Rabbit Polyclonal (IgG) to Human BUB1
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IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Detection of Human Bub1 by Immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-Bub1 used at a dilution of 1:1000 (1 ug/ml).

100% Guaranteed
Mouse Monoclonal to Human BUB1
Immunofluorescence, Western blot, Immunoprecipitation


Human BUB1
Human (tested or 100% immunogen sequence identity)
Protein A purified


  • Immunofluorescence
  • Western blot
  • Immunoprecipitation

Specificity and Use

BUB1 antibody was raised against recombinant human Bub1.
Amino acid residues 281-419 of human BUB1 protein.
This protein A purified antibody has been tested for use in immunoprecipitation, immunofluorescence staining and western blot and is capable of detecting endogenous protein. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band at ~160 kD corresponding to full-length protein by western blotting in the appropriate cell lysate or extract. The use of HeLa whole cell lysates prepared using a RIPA buffer is recommended as a positive control. For IF microscopy use cells grown on cover slips fixed with 3.5% paraformaldehyde in PBS at pH 6.8 OR 100% methanol at -20? C. Permeabilize fixed cells with 0.5% Triton X-100.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Store vial at -20 C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20 C or below. Avoid cycles of freezing and thawing.
For research use only.

About BUB1

O43683 NM_004336 NP_004327.1

BUB1 Antibody, Bub1A Antibody, BUB1L Antibody, HBUB1 Antibody

Serine/threonine-protein kinase that performs 2 crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Has a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of CENPF, BUB1B, CENPE and MAD2L1. Required for the kinetochore localization of PLK1. Plays an important role in defining SGOL1 localization and thereby affects sister chromatid cohesion.

Requested From: 
Date Requested: 4/25/2018

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