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Anti-BUB1 Antibody (aa281-419) LS-C18935

Ordering

Wt. Vol. Conc. Price
100 µg - - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular BUB1 Antibodies

Anti-BUB1 Antibody (N-Terminus) IHC-plus™ LS-B2108
Rabbit Polyclonal (IgG) to Human BUB1
Human, Mouse
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-BUB1 Antibody (aa1-30) LS-C156894
Rabbit Polyclonal to Human BUB1
Human
Western blot, Flow Cytometry
Unconjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal to Human BUB1
Human
Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated

Details

Human BUB1
Mouse
Human (tested or 100% immunogen sequence identity)
Monoclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • Immunofluorescence
  • Western blot
  • Immunoprecipitation

Specificity and Use

BUB1 antibody was raised against recombinant human Bub1.
aa281-419
Amino acid residues 281-419 of human BUB1 protein.
This protein A purified antibody has been tested for use in immunoprecipitation, immunofluorescence staining and western blot and is capable of detecting endogenous protein. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band at ~160 kD corresponding to full-length protein by western blotting in the appropriate cell lysate or extract. The use of HeLa whole cell lysates prepared using a RIPA buffer is recommended as a positive control. For IF microscopy use cells grown on cover slips fixed with 3.5% paraformaldehyde in PBS at pH 6.8 OR 100% methanol at -20? C. Permeabilize fixed cells with 0.5% Triton X-100.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Store vial at -20 C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20 C or below. Avoid cycles of freezing and thawing.
For research use only.

About BUB1

O43683 NM_004336 NP_004327.1

BUB1 Antibody, Bub1A Antibody, BUB1L Antibody, HBUB1 Antibody

Serine/threonine-protein kinase that performs 2 crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Has a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of CENPF, BUB1B, CENPE and MAD2L1. Required for the kinetochore localization of PLK1. Plays an important role in defining SGOL1 localization and thereby affects sister chromatid cohesion.

Requested From: United States
Date Requested: 12/3/2016

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