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Anti-BIK Antibody (N-Terminus) IHC-plus™ LS-B454

Note: This antibody replaces LS-C19604, LS-C56791, LS-C42486

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml Unavailable

Related Products

67 BIK Antibodies

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Anti-BIK Antibody (N-Terminus) IHC-plus™ LS-B454
Rabbit Polyclonal to Human BIK
Human, Mouse
IHC - Paraffin, ICC, Western blot
Unconjugated
Immunohistochemistry Image
Anti-BIK Antibody (aa39-74) LS-C98862
Rabbit Polyclonal to Human BIK
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Immunohistochemistry Image
Anti-BIK Antibody (clone 4G12) IHC-plus™ LS-B6011
Mouse Monoclonal [clone 4G12] (IgG) to Human BIK
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IHC - Paraffin, Western blot, ELISA
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Anti-BIK Antibody LS-C148238
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Human, Mouse, Rat, Gerbil
IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
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Immunohistochemistry Image
Anti-BIK Antibody (Met27) LS-C175937
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100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human BIK
Human, Mouse
IHC - Paraffin, ICC, Western blot
Unconjugated

Details

Human BIK
Rabbit
Human, Mouse (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • ICC
  • Western blot

Specificity and Use

BIK antibody was raised against synthetic peptide from human BIK.
LS-E30242 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-B454. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
N-Terminus
15 amino acid peptide from near the amino terminus of human BIK
Immunohistochemistry: LS-B454 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B454 was determined to be 5 ug/ml.

Packaging

PBS, 0.02% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
For research use only.

About BIK

Q13323 NM_001197 NP_001188.1

BIK Antibody, Apoptosis inducer NBK Antibody, Bcl-2-interacting killer Antibody, BP4 Antibody, Apoptosis-inducing NBK Antibody, BIP1 Antibody, NBK Antibody

BIK shares a critical BH3 domain with other death-promoting proteins, such as BID, BAK, BAD and BAX, that is required for its pro-apoptotic activity, and for interaction with anti-apoptotic members of the BCL2 family, and viral survival-promoting proteins. Since the activity of this protein is suppressed in the presence of survival-promoting proteins, it is suggested as a likely target for anti-apoptotic proteins.

Immunohistochemistry

Immunohistochemistry
Anti-BIK antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B454 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry
Immunocytochemistry of BIK in Jurkat cells with BIK antibody at 1 ug/ml.

Immunofluorescence

Immunofluorescence
Immunofluorescence of Bik in Jurkat cells with Bik antibody at 10 ug/ml.

Western blot

Western blot
Western blot of BIK in Jurkat cell lysate with BIK antibody at (A) 1 and (B) 2 ug/ml.

Requested From: 
Date Requested: 4/26/2017

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